Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | increasing DTT concentration results in increasing PcpC activity, reaching a maximum at approximately 30 mM DTT and remaining high up to 100 mM DTT | Sphingobium sp. UG30 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium sp. UG30 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | PcpC is not inhibited by the 2,3,5,6-tetrachlorohydroquinone substrate at concentrations 0.3 mM | Sphingobium sp. UG30 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0203 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 | |
0.1492 | - |
glutathione | pH 8.7, 50°C | Sphingobium sp. UG30 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28245 | - |
calculated from sequence | Sphingobium sp. UG30 |
30000 | - |
SDS-PAGE | Sphingobium sp. UG30 |
56000 | - |
gel filtration | Sphingobium sp. UG30 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. UG30 | Q937X0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium sp. UG30 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
103 | - |
pH 8.7, 50°C | Sphingobium sp. UG30 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Sphingobium sp. UG30 |
Synonyms | Comment | Organism |
---|---|---|
pcpC | - |
Sphingobium sp. UG30 |
tetrachlorohydroquinone reductive dehalogenase | - |
Sphingobium sp. UG30 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sphingobium sp. UG30 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 30% of maximal activity, 50°C: about 60% of maximal activity | Sphingobium sp. UG30 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, complete inactivation | Sphingobium sp. UG30 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.5 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Sphingobium sp. UG30 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | pH 8.0: about 50% of maximal activity, pH 9.5: about 40% of maximal activity | Sphingobium sp. UG30 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sphingobium sp. UG30 | calculated from sequence | - |
4.99 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2389 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 50°C | Sphingobium sp. UG30 |