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Literature summary for 1.21.4.3 extracted from

  • Hormann, K.; Andreesen, J.R.
    Reductive cleavage of sarcosine and betaine by Eubacterium acidaminophilum via enzyme systems different from glycine reductase (1989), Arch. Microbiol., 153, 50-59.
No PubMed abstract available
Search for more literature for 1.21.4.3

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol can serve as artificial electron donor Peptoclostridium acidaminophilum

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine 20% inhibition at 1 mM Peptoclostridium acidaminophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 N-methylglycine pH 7.7, 37°C Peptoclostridium acidaminophilum
11
-
 dithioerythritol pH 7.7, 37°C Peptoclostridium acidaminophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Peptoclostridium acidaminophilum 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-methylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum involved in creatinine and creatine metabolism, intermediate of choline degradation acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?

Organism

Organism UniProt Comment Textmining
Peptoclostridium acidaminophilum
-
 ATCC 49065, DSM 3953, expression of enzyme is induced only when grown on sarcosine or creatine
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
 pH 7.7, 37°C, extract from bacteria that were grown on formate and creatine Peptoclostridium acidaminophilum
217
-
 pH 7.7, 37°C, extract from bacteria that were grown on formate and sarcosine Peptoclostridium acidaminophilum

Storage Stability

Storage Stability Organism
-20°C, 15% loss of activity within one week Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methylglycine + phosphate + dithioerythritol
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + dithioerythritol disulfide
-
 ?
N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
N-methylglycine + phosphate + thioredoxin involved in creatinine and creatine metabolism, intermediate of choline degradation Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Peptoclostridium acidaminophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 8 highest activity in K-phosphate buffer Peptoclostridium acidaminophilum

Cofactor

Cofactor Comment Organism Structure
NADH 5% of activity compared to NADPH Peptoclostridium acidaminophilum
NADPH preferred as physiological electron donor Peptoclostridium acidaminophilum