Cloned (Comment) | Organism |
---|---|
cloning of two subunits of protein C: grdC1 and grdD1, expression in Escherichia coli | Peptoclostridium acidaminophilum |
Protein Variants | Comment | Organism |
---|---|---|
C359A | grdD of protein component C, mutant enzyme completely inactive, accessible to iodoacetate only under native conditions, suggesting that Cys359 of GrdD is the thiol responsible for the formation of the acetyl thioester during catalysis of arsenate-dependent hydrolysis of acetyl phosphate | Peptoclostridium acidaminophilum |
C98S | grdD of protein component C, activity is unchanged, accessible to iodoacetate only after denaturation | Peptoclostridium acidaminophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited, but protein C is protected from inactivation by treatment acetyl phosphate | Peptoclostridium acidaminophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Peptoclostridium acidaminophilum | - |
glycine + phosphate + thioredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peptoclostridium acidaminophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of protein C of enzyme, recombinant enzyme | Peptoclostridium acidaminophilum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | GrdD of protein component C catalyses the arsenate-dependent decomposition of acetyl phosphate, whereas GrdC completely inactive | Peptoclostridium acidaminophilum | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | Cys 359 of GrdD is the thiol responsible for the formation of the acetyl thioester during catalysis of arsenate-dependent hydrolysis of acetyl phosphate | Peptoclostridium acidaminophilum | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Peptoclostridium acidaminophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Peptoclostridium acidaminophilum | glycine + phosphate + thioredoxin | - |
? |