BRENDA - Enzyme Database show
show all sequences of 1.21.4.1

Studies on the enzymic reduction of amino acids. II. Purification and properties of a D-proline reductase and a proline racemase from Clostridium sticklandii

Stadtman, T.C.; Elliott, P.; J. Biol. Chem. 228, 983-997 (1957)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NAD+
-
Acetoanaerobium sticklandii
General Stability
General Stability
Organism
withstands ethanol and acetone fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Inhibitors
Inhibitors
Commentary
Organism
Structure
alkaline phosphomonoesterase
preparation from calf intestine, causes extensive inactivation
Acetoanaerobium sticklandii
arsenite
-
Acetoanaerobium sticklandii
Co2+
-
Acetoanaerobium sticklandii
Delta-Aminovalerate
-
Acetoanaerobium sticklandii
Fe2+
-
Acetoanaerobium sticklandii
human seminal plasma
50% inactivation by incubation at pH 5.5 for 90 min
Acetoanaerobium sticklandii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
dialyzed enzyme stimulated by
Acetoanaerobium sticklandii
Mg2+
depend on
Acetoanaerobium sticklandii
Mn2+
dialyzed enzyme stimulated by
Acetoanaerobium sticklandii
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Acetone
withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Ethanol
withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetoanaerobium sticklandii
-
formerly referred to as 'an amino acid-fermenting Clostridium, strain HF'
-
Clostridium sporogenes
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.4
-
-
Acetoanaerobium sticklandii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-allohydroxyproline + dihydrolipoate
barely detectable activity
2121
Acetoanaerobium sticklandii
5-amino-4-hydroxypentanoic acid + lipoate
-
-
-
ir
D-proline + 1,3-dimercaptopropanol
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + 1,3-dimercaptopropanol
oxidation of 1,3-dimercaptopropanol cannot be replaced by molecular hydrogen, NADH+ or a number of other reducing agents
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + 6,8-dimercaptooctanoate
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + beta-mercaptoethanol
-
2121
Acetoanaerobium sticklandii
?
-
-
-
ir
D-proline + dihydrolipoate
-
2121
Acetoanaerobium sticklandii
delta-aminovaleric acid + lipoate
-
-
-
ir
D-proline + dihydrolipoate
-
2121
Clostridium sporogenes
delta-aminovaleric acid + lipoate
-
-
-
ir
D-proline + dithiol (reduced)
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + oxidized dithiol
-
2121
Acetoanaerobium sticklandii
ir
D-proline + DL-lipoic acid
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NAD+
-
Acetoanaerobium sticklandii
General Stability (protein specific)
General Stability
Organism
withstands ethanol and acetone fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alkaline phosphomonoesterase
preparation from calf intestine, causes extensive inactivation
Acetoanaerobium sticklandii
arsenite
-
Acetoanaerobium sticklandii
Co2+
-
Acetoanaerobium sticklandii
Delta-Aminovalerate
-
Acetoanaerobium sticklandii
Fe2+
-
Acetoanaerobium sticklandii
human seminal plasma
50% inactivation by incubation at pH 5.5 for 90 min
Acetoanaerobium sticklandii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
dialyzed enzyme stimulated by
Acetoanaerobium sticklandii
Mg2+
depend on
Acetoanaerobium sticklandii
Mn2+
dialyzed enzyme stimulated by
Acetoanaerobium sticklandii
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Acetone
withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Ethanol
withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis
Acetoanaerobium sticklandii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.4
-
-
Acetoanaerobium sticklandii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-allohydroxyproline + dihydrolipoate
barely detectable activity
2121
Acetoanaerobium sticklandii
5-amino-4-hydroxypentanoic acid + lipoate
-
-
-
ir
D-proline + 1,3-dimercaptopropanol
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + 1,3-dimercaptopropanol
oxidation of 1,3-dimercaptopropanol cannot be replaced by molecular hydrogen, NADH+ or a number of other reducing agents
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + 6,8-dimercaptooctanoate
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
D-proline + beta-mercaptoethanol
-
2121
Acetoanaerobium sticklandii
?
-
-
-
ir
D-proline + dihydrolipoate
-
2121
Acetoanaerobium sticklandii
delta-aminovaleric acid + lipoate
-
-
-
ir
D-proline + dihydrolipoate
-
2121
Clostridium sporogenes
delta-aminovaleric acid + lipoate
-
-
-
ir
D-proline + dithiol (reduced)
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + oxidized dithiol
-
2121
Acetoanaerobium sticklandii
ir
D-proline + DL-lipoic acid
-
2121
Acetoanaerobium sticklandii
5-aminopentanoic acid + ?
-
-
-
ir
Other publictions for EC 1.21.4.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727777
Bouillaut
Proline-dependent regulation o ...
Clostridioides difficile, Clostridioides difficile 630
J. Bacteriol.
195
844-854
2013
-
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4
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-
1
1
1
1
-
-
704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
-
-
-
-
-
-
-
-
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-
1
-
1
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1
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-
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-
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-
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-
1
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
484935
Bednarski
In vitro processing of the pro ...
Acetoanaerobium sticklandii
Eur. J. Biochem.
268
3538-3544
2001
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
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-
1
1
-
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-
-
-
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-
1
-
-
-
-
-
-
-
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-
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2
-
-
-
-
1
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
484934
Kabisch
Identification of D-proline re ...
Acetoanaerobium sticklandii, Acetoanaerobium sticklandii DSM 519, Clostridium sporogenes
J. Biol. Chem.
274
8445-8454
1999
-
-
1
-
-
-
-
-
2
2
5
2
-
4
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
2
5
2
-
-
-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
484933
Arkowitz
The fate of the carboxyl oxyge ...
Acetoanaerobium sticklandii
Arch. Biochem. Biophys.
311
457-459
1994
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-
-
-
-
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-
1
-
1
-
-
1
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1
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1
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1
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1
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1
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1
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1
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1
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-
484932
Schwartz
-
D-Proline reductase from Clost ...
Acetoanaerobium sticklandii
FEMS Microbiol. Lett.
7
153-156
1980
2
-
-
-
-
-
-
-
-
7
-
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-
1
-
-
1
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-
2
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-
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-
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2
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-
7
-
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-
1
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2
-
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-
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-
484930
Schwartz
NADH-dependent reduction of D- ...
Acetoanaerobium sticklandii
Arch. Microbiol.
123
203-208
1979
-
-
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-
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-
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1
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3
-
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1
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2
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1
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1
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1
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1
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2
-
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-
-
-
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-
-
-
-
484931
Seto
Proline reductase: a sensitive ...
Acetoanaerobium sticklandii
Anal. Biochem.
95
44-47
1979
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
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1
-
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1
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1
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1
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1
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1
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1
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484929
Seto
Purification and properties of ...
Acetoanaerobium sticklandii
J. Biol. Chem.
251
2435-2439
1976
-
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-
-
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-
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-
1
-
3
1
-
1
-
-
1
-
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1
-
3
1
-
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1
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1
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1
-
3
1
-
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1
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1
-
3
1
-
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-
-
484928
Abeles
-
D-Proline reductase (Clostridi ...
Acetoanaerobium sticklandii
Methods Enzymol.
17
317-321
1971
1
-
-
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-
1
5
-
-
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-
1
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1
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1
2
1
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1
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1
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1
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5
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1
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1
2
1
-
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1
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-
484927
Hodgins
Studies of the mechanism of ac ...
Acetoanaerobium sticklandii
Arch. Biochem. Biophys.
130
274-285
1969
1
-
-
-
-
-
12
-
-
-
-
-
-
1
-
-
1
-
-
-
1
1
-
-
-
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1
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12
-
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1
-
-
1
1
-
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-
-
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-
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-
-
-
-
-
-
-
-
2121
Stadtman
Studies on the enzymic reducti ...
Acetoanaerobium sticklandii, Clostridium sporogenes
J. Biol. Chem.
228
983-997
1957
1
-
-
-
-
1
6
-
-
3
-
-
2
4
-
-
1
-
-
-
1
-
9
-
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-
-
-
-
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-
1
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1
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6
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3
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2
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1
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1
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9
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