Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | 5fold stimulation | Flavobacterium sp. | |
dithioerythritol | absolutely required, highly stimulating | Flavobacterium sp. | |
O2 | required | Flavobacterium sp. | |
polyethylene glycol 1500 | 20% stimulation up to concentration of 5% | Flavobacterium sp. | |
Triton X-100 | leads to 50% stimulation at concentration of 0.01% | Flavobacterium sp. |
Application | Comment | Organism |
---|---|---|
pharmacology | model system for study of endogenous functions of beta-lactams in bacteria | Flavobacterium sp. |
General Stability | Organism |
---|---|
unstable to 20 mM dithiothreitol during storage at -20°C, 10% activity remaining | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | moderate inhibition | Flavobacterium sp. | |
cystamine | 100% inhibition at 0.5 mM | Flavobacterium sp. | |
cysteamine | 43% inhibition at 1.5 mM | Flavobacterium sp. | |
cysteine | 60% inhibition at 1 mM | Flavobacterium sp. | |
cystine | 81% inhibition at 1.5 mM | Flavobacterium sp. | |
GSH | 70% inhibition at 1 mM | Flavobacterium sp. | |
Mn2+ | moderate inhibition | Flavobacterium sp. | |
additional information | ATP, NADPH and FAD: no effect; cations Na+, K+, Mg2+, Ca2+, Fe3+ do not affect the activity | Flavobacterium sp. | |
Ni2+ | moderate inhibition | Flavobacterium sp. | |
Triton X-100 | inhibits at concentration of 0.5% | Flavobacterium sp. | |
Zn2+ | moderate inhibition | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine | - |
Flavobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Flavobacterium sp. | 5829 | - |
membrane | inactive associated form | Flavobacterium sp. | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | stimulates | Flavobacterium sp. | |
Fe2+ | low concentration | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | 38000 | gel filtration and SDS-PAGE | Flavobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Flavobacterium sp. | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Flavobacterium sp. SC 12.154 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. SC 12.154 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00129 | - |
about, purified enzyme | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. | isopenicillin N + H2O | - |
r | |
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. SC 12.154 | isopenicillin N + H2O | - |
r | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Flavobacterium sp. | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. SC 12.154 | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Flavobacterium sp. SC 12.154 | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36500-38000, SDS-PAGE and gel filtration | Flavobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Flavobacterium sp. |
isopenicillin N-synthase | - |
Flavobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 20 | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 30% activity remaining above pH 9.0, highly reduced activity below pH 6.0 | Flavobacterium sp. |