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Literature summary for 1.21.1.2 extracted from

  • Romanov, V.; Hausinger, R.P.
    NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform Bacterium strain NBT-1 via 2,4-dichlorobenzoyl coenzym A (1996), J. Bacteriol., 178, 2656-2661.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.1.2

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol accumulation of 13% 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
glutathione accumulation of 14% 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
MgATP2- conversation of 2,4-dichlorobenzoyl-CoA in cytosolic fraction Clavibacter michiganensis subsp. sepedonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorobenzoyl-CoA + NADPH Clavibacter michiganensis subsp. sepedonicus
-
 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH Coryneform bacterium pathway for 2,4-dichlorobenzoate catabolism 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH Coryneform bacterium NTB-1 pathway for 2,4-dichlorobenzoate catabolism 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH Clavibacter michiganensis subsp. sepedonicus KZ-4
-
 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Clavibacter michiganensis subsp. sepedonicus
-
-
-
Clavibacter michiganensis subsp. sepedonicus KZ-4
-
-
-
Coryneform bacterium
-
 formerly named Alcaligenes denitrificans
-
Coryneform bacterium NTB-1
-
 formerly named Alcaligenes denitrificans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0008
-
-
 Clavibacter michiganensis subsp. sepedonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorobenzoyl-CoA + NADPH
-
 Clavibacter michiganensis subsp. sepedonicus 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH
-
 Coryneform bacterium 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH pathway for 2,4-dichlorobenzoate catabolism Coryneform bacterium 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH
-
 Coryneform bacterium NTB-1 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH pathway for 2,4-dichlorobenzoate catabolism Coryneform bacterium NTB-1 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?
2,4-dichlorobenzoyl-CoA + NADPH
-
 Clavibacter michiganensis subsp. sepedonicus KZ-4 4-chlorobenzoyl-CoA + NADP+ + chloride
-
 ?

Cofactor

Cofactor Comment Organism Structure
CoA conversion of 2,4-dichlorobenzoyl-CoA in cytosolic fraction Clavibacter michiganensis subsp. sepedonicus
NADH 10% accumulation of 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
NADH 2% accumulation of 4-chlorobenzoyl-CoA in cell extract Coryneform bacterium
NADPH 100% accumulation of 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
NADPH 100% accumulation of 4-chlorobenzoyl-CoA in cell extract Coryneform bacterium