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Literature summary for 1.20.99.1 extracted from
- Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite oxidase and its physiological electron acceptor (2004), Biochim. Biophys. Acta, 1656, 148-155.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
arsenite | pH 5.5, calculated with an Mr of 309000 Da | Hydrogenophaga sp. NT14 |  |
| 0.061 | - |
arsenite | pH 5.5 | Hydrogenophaga sp. NT-26 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | involved in catalysis, 10.6 mol per mol of alpha3beta3 | Hydrogenophaga sp. NT14 | |
| Iron | involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit | Hydrogenophaga sp. NT-26 | |
| Molybdenum | involved in catalysis, 2.1 mol per mol of alpha3beta3 | Hydrogenophaga sp. NT14 | |
| Molybdenum | involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit | Hydrogenophaga sp. NT-26 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE | Hydrogenophaga sp. NT-26 |
| 16000 | - |
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE | Hydrogenophaga sp. NT14 |
| 86000 | - |
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE | Hydrogenophaga sp. NT14 |
| 98000 | - |
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE | Hydrogenophaga sp. NT-26 |
| 219000 | - |
gel filtration | Hydrogenophaga sp. NT-26 |
| 306000 | - |
gel filtration | Hydrogenophaga sp. NT14 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenite + cytochrome c-551 | Hydrogenophaga sp. NT14 | the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth | arsenate + reduced cytochrome c-551 | - |
? | |
| arsenite + cytochrome c-551 | Hydrogenophaga sp. NT-26 | the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth | arsenate + reduced cytochrome c-551 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenophaga sp. NT-26 | - |
- |
- |
| Hydrogenophaga sp. NT14 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 57.5fold to homogeneity from the periplasm by preparation of spheroplasts, ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography, and gel filtration, purification via anion exchange or hydrophobic interaction chromatography is not successful | Hydrogenophaga sp. NT14 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| arsenite + acceptor + H2O = arsenate + reduced acceptor | electron transport chain, overview | Hydrogenophaga sp. NT14 | |
| arsenite + acceptor + H2O = arsenate + reduced acceptor | electron transport chain, overview | Hydrogenophaga sp. NT-26 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
purified enzyme | Hydrogenophaga sp. NT14 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenite + cytochrome c-551 | the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth | Hydrogenophaga sp. NT14 | arsenate + reduced cytochrome c-551 | - |
? | |
| arsenite + cytochrome c-551 | the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth | Hydrogenophaga sp. NT-26 | arsenate + reduced cytochrome c-551 | - |
? | |
| arsenite + cytochrome c-551 | electron transport chain | Hydrogenophaga sp. NT14 | arsenate + cytochrome c-551 | - |
? | |
| arsenite + cytochrome c-551 | electron transport chain, overview | Hydrogenophaga sp. NT-26 | arsenate + cytochrome c-551 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE | Hydrogenophaga sp. NT14 |
| tetramer | 2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE | Hydrogenophaga sp. NT-26 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arsenite oxidase | - |
Hydrogenophaga sp. NT14 |
| arsenite oxidase | - |
Hydrogenophaga sp. NT-26 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30.4 | - |
arsenite | pH 5.5, calculated with an Mr of 309000 Da | Hydrogenophaga sp. NT14 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Hydrogenophaga sp. NT14 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c-551 | purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer | Hydrogenophaga sp. NT14 | |
| cytochrome c-551 | purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer | Hydrogenophaga sp. NT-26 |
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