BRENDA - Enzyme Database show
show all sequences of 1.20.9.1

Purification and characterization of arsenite oxidase from Arthrobacter sp.

Prasad, K.S.; Subramanian, V.; Paul, J.; Biometals 22, 711-721 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
1 mM, 1.5% residual activity
Arthrobacter sp.
diethyldicarbonate
1 mM, 5% residual activity
Arthrobacter sp.
additional information
not inhibitory: 1 mM of Na+, Ca2+, Fe2+, Mo6+ and EDTA
Arthrobacter sp.
Zn2+
1 mM, 1.8% residual activity
Arthrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
arsenite
pH 6.0
Arthrobacter sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
about 90% of enzyme activity within the membrane fraction
Arthrobacter sp.
16020
-
periplasm
about 10% of enzyme activity within the periplasmic space
Arthrobacter sp.
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
100000
-
PAGE
Arthrobacter sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + 2,6-dichlorophenol indophenol
-
696599
Arthrobacter sp.
arsenate + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Arthrobacter sp.
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5
35
more than 92% residual activity
Arthrobacter sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
1 h, 70% residual activity
Arthrobacter sp.
45
-
1 h, 55% residual activity
Arthrobacter sp.
50
-
1 h, 15% residual activity
Arthrobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arthrobacter sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
78% residual activity
Arthrobacter sp.
7
-
85% residual activity
Arthrobacter sp.
8
-
82% residual activity
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
1 mM, 1.5% residual activity
Arthrobacter sp.
diethyldicarbonate
1 mM, 5% residual activity
Arthrobacter sp.
additional information
not inhibitory: 1 mM of Na+, Ca2+, Fe2+, Mo6+ and EDTA
Arthrobacter sp.
Zn2+
1 mM, 1.8% residual activity
Arthrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
arsenite
pH 6.0
Arthrobacter sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
about 90% of enzyme activity within the membrane fraction
Arthrobacter sp.
16020
-
periplasm
about 10% of enzyme activity within the periplasmic space
Arthrobacter sp.
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
100000
-
PAGE
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + 2,6-dichlorophenol indophenol
-
696599
Arthrobacter sp.
arsenate + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Arthrobacter sp.
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5
35
more than 92% residual activity
Arthrobacter sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
1 h, 70% residual activity
Arthrobacter sp.
45
-
1 h, 55% residual activity
Arthrobacter sp.
50
-
1 h, 15% residual activity
Arthrobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arthrobacter sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
78% residual activity
Arthrobacter sp.
7
-
85% residual activity
Arthrobacter sp.
8
-
82% residual activity
Arthrobacter sp.
Other publictions for EC 1.20.9.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696599
Prasad
Purification and characterizat ...
Arthrobacter sp.
Biometals
22
711-721
2009
-
-
-
-
-
-
4
1
3
-
2
-
-
6
-
-
-
-
-
-
-
-
1
1
1
1
3
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
3
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
3
-
1
-
3
-
-
-
-
-
-
-
690420
Male
Biosensor for arsenite using a ...
Rhizobium sp.
Anal. Chem.
79
7831-7837
2007
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692109
Inskeep
Detection, diversity and expre ...
Achromobacter sp., Achromobacter sp. NT10, Achromobacter sp. WA20, Agrobacterium tumefaciens, Agrobacterium tumefaciens 5A, arsenite-oxidising alpha proteobacterium Ben-5, arsenite-oxidising alpha proteobacterium Ben-5 Ben5, arsenite-oxidising alpha proteobacterium NT-4, arsenite-oxidising beta proteobacterium NT-14, arsenite-oxidising beta proteobacterium WA13, arsenite-oxidising beta proteobacterium WA19, Hydrogenophaga sp. WA13, Mesorhizobium sp., Mesorhizobium sp. DM1, Rhizobium sp., Rhizobium sp. NT26, Sinorhizobium sp. NT4, Thermus sp., Thermus sp. HR13, Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579, Variovorax sp., Variovorax sp. RM1
Environ. Microbiol.
9
934-943
2007
1
12
-
-
-
-
-
-
-
12
-
-
-
25
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659786
Anderson
-
Metabolic energy from arsenite ...
Alcaligenes faecalis
J. Phys. IV Proc.
107
49-52
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287858
Ellis
Crystal structure of the 100 k ...
Alcaligenes faecalis, Alcaligenes faecalis NCIB 8687
Structure
9
125-132
2001
-
-
-
1
-
-
-
-
-
3
-
-
-
8
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287859
Krafft
Purification and characterizat ...
Chrysiogenes arsenatis
Eur. J. Biochem.
255
647-653
1998
-
-
-
-
-
-
-
1
1
3
3
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
3
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
287857
Anderson
The purification and character ...
Alcaligenes faecalis
J. Biol. Chem.
267
23674-23682
1992
-
-
-
-
-
-
-
2
-
2
1
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-