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Literature summary for 1.20.9.1 extracted from

  • Prasad, K.S.; Subramanian, V.; Paul, J.
    Purification and characterization of arsenite oxidase from Arthrobacter sp. (2009), Biometals, 22, 711-721.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 1.5% residual activity Arthrobacter sp.
diethyldicarbonate 1 mM, 5% residual activity Arthrobacter sp.
additional information not inhibitory: 1 mM of Na+, Ca2+, Fe2+, Mo6+ and EDTA Arthrobacter sp.
Zn2+ 1 mM, 1.8% residual activity Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
arsenite pH 6.0 Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane about 90% of enzyme activity within the membrane fraction Arthrobacter sp. 16020
-
periplasm about 10% of enzyme activity within the periplasmic space Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE Arthrobacter sp.
100000
-
PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenite + 2,6-dichlorophenol indophenol
-
Arthrobacter sp. arsenate + reduced 2,6-dichlorophenol indophenol
-
?

Subunits

Subunits Comment Organism
dimer 1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 35 more than 92% residual activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
1 h, 70% residual activity Arthrobacter sp.
45
-
1 h, 55% residual activity Arthrobacter sp.
50
-
1 h, 15% residual activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
78% residual activity Arthrobacter sp.
7
-
85% residual activity Arthrobacter sp.
8
-
82% residual activity Arthrobacter sp.