BRENDA - Enzyme Database show
show all sequences of 1.20.9.1

Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A

Ellis, P.J.; Conrads, T.; Hille, R.; Kuhn, P.; Structure 9, 125-132 (2001)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
; crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Iron
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Molybdenum
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis
Q7SIF3
small subunit aoxA
-
Alcaligenes faecalis
Q7SIF4
large subunit aoxB
-
Alcaligenes faecalis NCIB 8687
Q7SIF3
small subunit aoxA
-
Alcaligenes faecalis NCIB 8687
Q7SIF4
large subunit aoxB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + H2O + azurin(oxidized)
-
287858
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287858
Alcaligenes faecalis
ir
Subunits
Subunits
Commentary
Organism
More
heterodimer
Alcaligenes faecalis
Cofactor
Cofactor
Commentary
Organism
Structure
iron-sulfur centre
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
molybdopterin
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
iron-sulfur centre
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
molybdopterin
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Alcaligenes faecalis
crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Iron
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
Molybdenum
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + H2O + azurin(oxidized)
-
287858
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287858
Alcaligenes faecalis
ir
Subunits (protein specific)
Subunits
Commentary
Organism
More
heterodimer
Alcaligenes faecalis
Other publictions for EC 1.20.9.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696599
Prasad
Purification and characterizat ...
Arthrobacter sp.
Biometals
22
711-721
2009
-
-
-
-
-
-
4
1
3
-
2
-
-
6
-
-
-
-
-
-
-
-
1
1
1
1
3
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
3
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
3
-
1
-
3
-
-
-
-
-
-
-
690420
Male
Biosensor for arsenite using a ...
Rhizobium sp.
Anal. Chem.
79
7831-7837
2007
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692109
Inskeep
Detection, diversity and expre ...
Achromobacter sp., Achromobacter sp. NT10, Achromobacter sp. WA20, Agrobacterium tumefaciens, Agrobacterium tumefaciens 5A, arsenite-oxidising alpha proteobacterium Ben-5, arsenite-oxidising alpha proteobacterium Ben-5 Ben5, arsenite-oxidising alpha proteobacterium NT-4, arsenite-oxidising beta proteobacterium NT-14, arsenite-oxidising beta proteobacterium WA13, arsenite-oxidising beta proteobacterium WA19, Hydrogenophaga sp. WA13, Mesorhizobium sp., Mesorhizobium sp. DM1, Rhizobium sp., Rhizobium sp. NT26, Sinorhizobium sp. NT4, Thermus sp., Thermus sp. HR13, Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579, Variovorax sp., Variovorax sp. RM1
Environ. Microbiol.
9
934-943
2007
1
12
-
-
-
-
-
-
-
12
-
-
-
25
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659786
Anderson
-
Metabolic energy from arsenite ...
Alcaligenes faecalis
J. Phys. IV Proc.
107
49-52
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287858
Ellis
Crystal structure of the 100 k ...
Alcaligenes faecalis, Alcaligenes faecalis NCIB 8687
Structure
9
125-132
2001
-
-
-
1
-
-
-
-
-
3
-
-
-
8
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287859
Krafft
Purification and characterizat ...
Chrysiogenes arsenatis
Eur. J. Biochem.
255
647-653
1998
-
-
-
-
-
-
-
1
1
3
3
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
3
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
287857
Anderson
The purification and character ...
Alcaligenes faecalis
J. Biol. Chem.
267
23674-23682
1992
-
-
-
-
-
-
-
2
-
2
1
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-