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Literature summary for 1.20.4.3 extracted from

  • Eberle, R.J.; Kawai, L.A.; de Moraes, F.R.; Tasic, L.; Arni, R.K.; Coronado, M.A.
    Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis (2018), Int. J. Biol. Macromol., 107, 1999-2007 .
    View publication on PubMed
Search for more literature for 1.20.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium pseudotuberculosis

Inhibitors

Inhibitors Comment Organism Structure
CoA the enzyme activity is reduced by 80% in the presence of CoA Corynebacterium pseudotuberculosis
Diamide the enzyme activity is reduced by 95% in the presence of diamide Corynebacterium pseudotuberculosis
ergothioneine the enzyme activity is reduced by 10% in the presence of ergothioneine Corynebacterium pseudotuberculosis
GSH the enzyme activity is reduced by 60% in the presence of GSH Corynebacterium pseudotuberculosis
heparin the enzyme activity is reduced by about 92% in the presence of heparin Corynebacterium pseudotuberculosis
hesperetin the enzyme activity is reduced by about 75% in the presence of hesperitin Corynebacterium pseudotuberculosis
hesperidin the enzyme activity is reduced by about 87% in the presence of hesperidin Corynebacterium pseudotuberculosis
suramin the enzyme activity is reduced by about 87% in the presence of suramin Corynebacterium pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Corynebacterium pseudotuberculosis D9Q987
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Corynebacterium pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insulin + dithiothreitol
-
 Corynebacterium pseudotuberculosis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 11000, SDS-PAGE Corynebacterium pseudotuberculosis
? x * 11155, calculated from amino acid sequence Corynebacterium pseudotuberculosis

Synonyms

Synonyms Comment Organism
glutaredoxin A1
-
 Corynebacterium pseudotuberculosis
mycoredoxin
-
 Corynebacterium pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Corynebacterium pseudotuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
7 8 more than 60% activity between pH 7.0 and 8.0 Corynebacterium pseudotuberculosis