BRENDA - Enzyme Database show
show all sequences of 1.20.2.1

Molybdenum-containing arsenite oxidase of the chemolithoautotrophic arsenite oxidizer NT-26

Santini, J.; Vanden Hoven, R.; J. Bacteriol. 186, 1614-1619 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
arsenite
pH 5.5, temperature not specified in the publication
arsenite-oxidising bacterium
0.061
-
arsenite
pH 5.5, temperature not specified in the publication
Rhizobium sp. NT-26
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
arsenite-oxidising bacterium
-
-
periplasm
-
Rhizobium sp. NT-26
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
arsenite-oxidising bacterium
Iron
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
Rhizobium sp. NT-26
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
14000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
98000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
98000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
219000
-
gel filtration
arsenite-oxidising bacterium
219000
-
gel filtration
Rhizobium sp. NT-26
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
arsenite-oxidising bacterium
Q6VAL9
subunit aroB
-
arsenite-oxidising bacterium NT-26
Q6VAL9
subunit aroB
-
Rhizobium sp. NT-26
Q6VAL8
-
-
Purification (Commentary)
Commentary
Organism
-
arsenite-oxidising bacterium
-
Rhizobium sp. NT-26
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
Rhizobium sp. NT-26
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
arsenite-oxidising bacterium
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
arsenite-oxidising bacterium NT-26
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
tetramer
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.6
-
arsenite
pH 5.5, temperature not specified in the publication
arsenite-oxidising bacterium
8.6
-
arsenite
pH 5.5, temperature not specified in the publication
Rhizobium sp. NT-26
Cofactor
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
arsenite-oxidising bacterium
molybdenum cofactor
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
Rhizobium sp. NT-26
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
arsenite-oxidising bacterium
molybdenum cofactor
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
Rhizobium sp. NT-26
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
arsenite
pH 5.5, temperature not specified in the publication
arsenite-oxidising bacterium
0.061
-
arsenite
pH 5.5, temperature not specified in the publication
Rhizobium sp. NT-26
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
arsenite-oxidising bacterium
-
-
periplasm
-
Rhizobium sp. NT-26
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
arsenite-oxidising bacterium
Iron
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
Rhizobium sp. NT-26
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
14000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
98000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
98000
-
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
219000
-
gel filtration
arsenite-oxidising bacterium
219000
-
gel filtration
Rhizobium sp. NT-26
Purification (Commentary) (protein specific)
Commentary
Organism
-
arsenite-oxidising bacterium
-
Rhizobium sp. NT-26
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
Rhizobium sp. NT-26
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
arsenite-oxidising bacterium
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
715352
arsenite-oxidising bacterium NT-26
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
arsenite-oxidising bacterium
tetramer
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
Rhizobium sp. NT-26
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.6
-
arsenite
pH 5.5, temperature not specified in the publication
arsenite-oxidising bacterium
8.6
-
arsenite
pH 5.5, temperature not specified in the publication
Rhizobium sp. NT-26
General Information
General Information
Commentary
Organism
physiological function
an aroA disruption mutant does not display arsenite oxidation activity, fails to grow chemolithoautotrophically with arsenite as the electron donor, but is able to grow in presence of 5 mM arsenite
Rhizobium sp. NT-26
General Information (protein specific)
General Information
Commentary
Organism
physiological function
an aroA disruption mutant does not display arsenite oxidation activity, fails to grow chemolithoautotrophically with arsenite as the electron donor, but is able to grow in presence of 5 mM arsenite
Rhizobium sp. NT-26
Other publictions for EC 1.20.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727089
Kalimuthu
Electrochemically driven catal ...
Rhizobium sp.
Biochim. Biophys. Acta
1837
112-120
2014
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-
1
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-
1
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-
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1
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1
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1
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1
1
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1
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1
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-
-
-
-
713822
Branco
Identification of an aox syste ...
Ochrobactrum tritici, Ochrobactrum tritici SCII24
Appl. Environ. Microbiol.
75
5141-5147
2009
-
-
1
-
1
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
4
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1
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2
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2
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4
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-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
1
1
2
2
-
-
714842
Duquesne
Arsenite oxidation by a chemoa ...
Thiomonas sp., Thiomonas sp. 3AS
Environ. Microbiol.
10
228-237
2008
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-
-
-
-
-
-
-
1
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4
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1
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2
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3
-
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2
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-
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-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
714304
Santini
The NT-26 cytochrome c552 and ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26
Biochim. Biophys. Acta
1767
189-196
2007
-
-
-
-
-
-
-
-
-
-
-
2
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3
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4
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1
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1
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2
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4
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658195
vanden Hoven
Arsenite oxidation by the hete ...
Hydrogenophaga sp., Hydrogenophaga sp. NT-14
Biochim. Biophys. Acta
1656
148-155
2004
-
-
-
-
-
-
-
1
1
2
3
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
2
3
2
-
-
-
1
-
-
1
-
4
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
715352
Santini
Molybdenum-containing arsenite ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26, Rhizobium sp. NT-26
J. Bacteriol.
186
1614-1619
2004
-
-
-
-
-
-
-
2
2
2
6
-
-
4
-
-
2
-
-
-
-
-
3
2
-
-
-
2
-
-
-
2
-
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-
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-
2
-
-
-
-
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2
2
2
6
-
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-
2
-
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-
3
2
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-
2
-
-
-
-
-
1
1
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