BRENDA - Enzyme Database
show all sequences of 1.20.2.1

Identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium Ochrobactrum tritici SCII24

Branco, R.; Francisco, R.; Chung, A.; Morais, P.; Appl. Environ. Microbiol. 75, 5141-5147 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer; functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Engineering
Protein Variants
Commentary
Organism
additional information
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer; functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
no activity detected in membrane fraction; no activity detected in membrane fraction
Ochrobactrum tritici
-
-
soluble
;
Ochrobactrum tritici
-
-
Organism
Organism
UniProt
Commentary
Textmining
Ochrobactrum tritici
B7UB93
small subunit aoxA
-
Ochrobactrum tritici
B7UB94
large subunit aoxB
-
Ochrobactrum tritici SCII24
B7UB93
small subunit aoxA
-
Ochrobactrum tritici SCII24
B7UB94
large subunit aoxB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici SCII24
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
aoxA
-
Ochrobactrum tritici
aoxB
-
Ochrobactrum tritici
As(III) oxidase
-
Ochrobactrum tritici
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
;
Ochrobactrum tritici
Cloned(Commentary) (protein specific)
Commentary
Organism
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
no activity detected in membrane fraction
Ochrobactrum tritici
-
-
soluble
-
Ochrobactrum tritici
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici SCII24
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Ochrobactrum tritici
Expression
Organism
Commentary
Expression
Ochrobactrum tritici
coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR; coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR
additional information
General Information
General Information
Commentary
Organism
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon; the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon. An aoxB deletion mutant fails to oxidize As(III) to As(V), but is able to grow in the presence of high arsenite concentrations
Ochrobactrum tritici
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon. An aoxB deletion mutant fails to oxidize As(III) to As(V), but is able to grow in the presence of high arsenite concentrations
Ochrobactrum tritici
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon
Ochrobactrum tritici
Expression (protein specific)
Organism
Commentary
Expression
Ochrobactrum tritici
coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR
additional information
Other publictions for EC 1.20.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727089
Kalimuthu
Electrochemically driven catal ...
Rhizobium sp.
Biochim. Biophys. Acta
1837
112-120
2014
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
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-
-
-
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1
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-
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1
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1
1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713822
Branco
Identification of an aox syste ...
Ochrobactrum tritici, Ochrobactrum tritici SCII24
Appl. Environ. Microbiol.
75
5141-5147
2009
-
-
1
-
1
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
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4
-
4
-
-
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-
1
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-
-
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2
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2
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-
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4
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-
-
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4
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-
-
-
-
2
-
-
-
1
1
2
2
-
-
714842
Duquesne
Arsenite oxidation by a chemoa ...
Thiomonas sp., Thiomonas sp. 3AS
Environ. Microbiol.
10
228-237
2008
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-
-
-
-
-
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1
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4
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1
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2
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3
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2
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2
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1
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2
-
-
714304
Santini
The NT-26 cytochrome c552 and ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26
Biochim. Biophys. Acta
1767
189-196
2007
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-
-
-
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-
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-
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2
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3
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4
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1
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1
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2
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4
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-
-
-
-
658195
vanden Hoven
Arsenite oxidation by the hete ...
Hydrogenophaga sp., Hydrogenophaga sp. NT-14
Biochim. Biophys. Acta
1656
148-155
2004
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-
-
-
-
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-
1
1
2
3
2
-
2
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1
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1
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4
1
2
-
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1
1
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1
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1
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1
1
2
3
2
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1
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1
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4
1
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1
1
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-
-
-
-
715352
Santini
Molybdenum-containing arsenite ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26, Rhizobium sp. NT-26
J. Bacteriol.
186
1614-1619
2004
-
-
-
-
-
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-
2
2
2
6
-
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4
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2
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3
2
2
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2
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2
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2
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2
2
2
6
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2
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3
2
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2
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1
1
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