BRENDA - Enzyme Database show
show all sequences of 1.20.2.1

Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite oxidase and its physiological electron acceptor

vanden Hoven, R.N.; Santini, J.M.; Biochim. Biophys. Acta 1656, 148-155 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Hydrogenophaga sp.
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains 10.6 mol/alpha3beta3 iron and 2.1 mol/alpha3beta3 molybdenum
Hydrogenophaga sp.
Molybdenum
protein contains 10.6 mol iron per alpha3beta3 unit and 2.1 mol molybdenum per alpha3beta3 molybdenum
Hydrogenophaga sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
306000
-
gel filtration
Hydrogenophaga sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp.
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp. NT-14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hydrogenophaga sp.
-
-
-
Hydrogenophaga sp. NT-14
-
-
-
Purification (Commentary)
Commentary
Organism
-
Hydrogenophaga sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.6
-
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp.
arsenate + reduced cytochrome c-551
-
-
-
?
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-14
arsenate + reduced cytochrome c-551
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp.
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp. NT-14
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
30.4
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Hydrogenophaga sp.
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c-551
physiological electron acceptor for the arsenite oxidase
Hydrogenophaga sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c-551
physiological electron acceptor for the arsenite oxidase
Hydrogenophaga sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Hydrogenophaga sp.
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains 10.6 mol/alpha3beta3 iron and 2.1 mol/alpha3beta3 molybdenum
Hydrogenophaga sp.
Molybdenum
protein contains 10.6 mol iron per alpha3beta3 unit and 2.1 mol molybdenum per alpha3beta3 molybdenum
Hydrogenophaga sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
306000
-
gel filtration
Hydrogenophaga sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp.
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp. NT-14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hydrogenophaga sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.6
-
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp.
arsenate + reduced cytochrome c-551
-
-
-
?
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-14
arsenate + reduced cytochrome c-551
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp.
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp. NT-14
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
30.4
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Hydrogenophaga sp.
Other publictions for EC 1.20.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727089
Kalimuthu
Electrochemically driven catal ...
Rhizobium sp.
Biochim. Biophys. Acta
1837
112-120
2014
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713822
Branco
Identification of an aox syste ...
Ochrobactrum tritici, Ochrobactrum tritici SCII24
Appl. Environ. Microbiol.
75
5141-5147
2009
-
-
1
-
1
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
1
1
2
2
-
-
714842
Duquesne
Arsenite oxidation by a chemoa ...
Thiomonas sp., Thiomonas sp. 3AS
Environ. Microbiol.
10
228-237
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
714304
Santini
The NT-26 cytochrome c552 and ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26
Biochim. Biophys. Acta
1767
189-196
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658195
vanden Hoven
Arsenite oxidation by the hete ...
Hydrogenophaga sp., Hydrogenophaga sp. NT-14
Biochim. Biophys. Acta
1656
148-155
2004
-
-
-
-
-
-
-
1
1
2
3
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
2
3
2
-
-
-
1
-
-
1
-
4
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
715352
Santini
Molybdenum-containing arsenite ...
arsenite-oxidising bacterium, arsenite-oxidising bacterium NT-26, Rhizobium sp. NT-26
J. Bacteriol.
186
1614-1619
2004
-
-
-
-
-
-
-
2
2
2
6
-
-
4
-
-
2
-
-
-
-
-
3
2
-
-
-
2
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
2
6
-
-
-
-
2
-
-
-
-
3
2
-
-
-
2
-
-
-
-
-
1
1
-
-
-