Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
analysis of the electrostatic potentials of the interfaces between the subunits. Regions A, B, and C are almost electroneutral | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, no residual activity | Pseudomonas sp. | |
Cu2+ | 1 mM, about 55% of initial activity | Pseudomonas sp. | |
Mn2+ | 1 mM, about 65% of initial activity | Pseudomonas sp. | |
Zn2+ | 1 mM, about 35% of initial activity | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NAD+ | pH 7.0, 25°C | Pseudomonas sp. | |
0.475 | - |
phosphite | pH 7.0, 25°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, about 155% of initial activity | Pseudomonas sp. | |
Fe3+ | 1 mM, about 150% of initial activity | Pseudomonas sp. | |
Mg2+ | 1 mM, about 125% of initial activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Pseudomonas sp. |
70000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | F2YGD2 | - |
- |
Pseudomonas sp. K | F2YGD2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.49 | - |
pH 7.0, 25°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphite + H2O + NAD+ | - |
Pseudomonas sp. | phosphate + NADH + H+ | - |
? | |
phosphite + H2O + NAD+ | - |
Pseudomonas sp. K | phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
about 13% of maximum activity | Pseudomonas sp. |
55 | - |
less than 10% of maximum activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Pseudomonas sp. |
45 | - |
hlf-life 35 min | Pseudomonas sp. |
55 | - |
complete inactivation | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
NaH2PO4/H3PO4 buffer | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 10.5 | - |
Pseudomonas sp. |
7 | - |
assay at, activity at pH 3.0 is about 2.2fold higher than at pH 7.0 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | 1 h, more than 80% residual activity | Pseudomonas sp. |