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Literature summary for 1.20.1.1 extracted from

  • Liu, D.F.; Ding, H.T.; Du, Y.Q.; Zhao, Y.H.; Jia, X.M.
    Cloning, expression, and characterization of a wide-pH-range stable phosphite dehydrogenase from Pseudomonas sp. K in Escherichia coli (2012), Appl. Biochem. Biotechnol., 166, 1301-1313.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
analysis of the electrostatic potentials of the interfaces between the subunits. Regions A, B, and C are almost electroneutral Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, no residual activity Pseudomonas sp.
Cu2+ 1 mM, about 55% of initial activity Pseudomonas sp.
Mn2+ 1 mM, about 65% of initial activity Pseudomonas sp.
Zn2+ 1 mM, about 35% of initial activity Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NAD+ pH 7.0, 25°C Pseudomonas sp.
0.475
-
phosphite pH 7.0, 25°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
EDTA 1 mM, about 155% of initial activity Pseudomonas sp.
Fe3+ 1 mM, about 150% of initial activity Pseudomonas sp.
Mg2+ 1 mM, about 125% of initial activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Pseudomonas sp.
70000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. F2YGD2
-
-
Pseudomonas sp. K F2YGD2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.49
-
pH 7.0, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphite + H2O + NAD+
-
Pseudomonas sp. phosphate + NADH + H+
-
?
phosphite + H2O + NAD+
-
Pseudomonas sp. K phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
about 13% of maximum activity Pseudomonas sp.
55
-
less than 10% of maximum activity Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable below Pseudomonas sp.
45
-
hlf-life 35 min Pseudomonas sp.
55
-
complete inactivation Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
NaH2PO4/H3PO4 buffer Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
2.5 10.5
-
Pseudomonas sp.
7
-
assay at, activity at pH 3.0 is about 2.2fold higher than at pH 7.0 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 1 h, more than 80% residual activity Pseudomonas sp.