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Literature summary for 1.20.1.1 extracted from

  • Relyea, H.A.; van der Donk, W.A.
    Mechanism and applications of phosphite dehydrogenase (2005), Bioorg. Chem., 33, 171-189.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of deuterium- or tritium-labeled substances, mutant enzymes could be applied as NADPH regeneration systems Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
E175A/A176R double mutant, 3.6-fold higher efficiency with NAD+, 1000-fold higher efficiency with NADP+, 3-fold favor for NADP+ over NAD+ as cofactor Pseudomonas stutzeri
H292F no activity, NAD+ binding is abolished Pseudomonas stutzeri
H292K no activity, NAD+ binding is abolished Pseudomonas stutzeri
H292N no activity, NAD+ binding is abolished Pseudomonas stutzeri
H292Q no activity, NAD+ binding is abolished Pseudomonas stutzeri
R237K strong increase in Km for both substrates, reduced Vmax Pseudomonas stutzeri

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate
-
Pseudomonas stutzeri
sulfite strong inhibitor, competitive with respect to posphonate Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphonate + H2O + NAD+ Alcaligenes faecalis NADH regeneration system phosphate + NADH
-
ir
phosphonate + H2O + NAD+ Pseudomonas stutzeri NADH regeneration system phosphate + NADH
-
ir
phosphonate + H2O + NAD+ Pseudomonas stutzeri WM88 NADH regeneration system phosphate + NADH
-
ir

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri WM88
-
-
-

Reaction

Reaction Comment Organism Reaction ID
phosphonate + NAD+ + H2O = phosphate + NADH + H+ different reaction mechanism discussed Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme does not accept any alternative substrates Pseudomonas stutzeri ?
-
?
additional information enzyme does not accept any alternative substrates Pseudomonas stutzeri WM88 ?
-
?
phosphonate + H2O + NAD+
-
Alcaligenes faecalis phosphate + NADH
-
ir
phosphonate + H2O + NAD+
-
Pseudomonas stutzeri phosphate + NADH
-
ir
phosphonate + H2O + NAD+ NADH regeneration system Alcaligenes faecalis phosphate + NADH
-
ir
phosphonate + H2O + NAD+ NADH regeneration system Pseudomonas stutzeri phosphate + NADH
-
ir
phosphonate + H2O + NAD+
-
Pseudomonas stutzeri WM88 phosphate + NADH
-
ir
phosphonate + H2O + NAD+ NADH regeneration system Pseudomonas stutzeri WM88 phosphate + NADH
-
ir
phosphonate + H2O + NADP+ NADP+ is a very poor hydride acceptor with the wild type enzyme Pseudomonas stutzeri phosphate + NADPH
-
ir
phosphonate + H2O + NADP+ NADP+ is a very poor hydride acceptor with the wild type enzyme Pseudomonas stutzeri WM88 phosphate + NADPH
-
ir

Synonyms

Synonyms Comment Organism
PTDH
-
Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas stutzeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.25
-
-
Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Alcaligenes faecalis
NAD+
-
Pseudomonas stutzeri
NADP+
-
Pseudomonas stutzeri