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Literature summary for 1.2.99.7 extracted from

  • Kozono, I.; Hibi, M.; Takeuchi, M.; Ogawa, J.
    Purification and characterization of molybdenum-containing aldehyde dehydrogenase that oxidizes benzyl maltol derivative from Pseudomonas nitroreducens SB32154 (2020), Biosci. Biotechnol. Biochem., 84, 2390-2400 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
2-nitrobenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
0.22
-
3-Nitrobenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
0.35
-
4-nitrobenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
0.75
-
4-hydroxybenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
1
-
2-Hydroxybenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
1.1
-
3-Hydroxybenzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
1.5
-
cinnamaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
7
-
benzaldehyde pH 6, 30┬░C Pseudomonas nitroreducens
24
-
3-(benzyloxy)-4-oxo-4H-pyran-2-carbaldehyde pH 6, 30┬░C Pseudomonas nitroreducens

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Pseudomonas nitroreducens

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens A0A5K7VLU0 and A0A5K7VFT8 A0A5K7VLU0 i.e. small subunit, A0A5K7VFT8 i.e. large subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 2-hydroxybenzylic acid + reduced phenyazine methosulfate
-
?
2-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 2-nitrobenzylic acid + reduced phenyazine methosulfate
-
?
3-(benzyloxy)-4-oxo-4H-pyran-2-carbaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 3-(benzyloxy)-4-oxo-4H-pyran-2-carboxylic acid + reduced phenyazine methosulfate
-
?
3-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 3-hydroxybenzylic acid + reduced phenyazine methosulfate
-
?
3-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 3-nitrobenzylic acid + reduced phenyazine methosulfate
-
?
4-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 4-hydroxybenzylic acid + reduced phenyazine methosulfate
-
?
4-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens 4-nitrobenzoic acid + reduced phenyazine methosulfate
-
?
benzaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens benzylic acid + reduced phenyazine methosulfate
-
?
cinnamaldehyde + H2O + phenyazine methosulfate
-
Pseudomonas nitroreducens cinnamic acid + reduced phenyazine methosulfate
-
?
additional information additional albeit significantly less effective acdeptors are dichlorophenolindophenol, methylene blue, ferricyanide Pseudomonas nitroreducens ?
-
-

Cofactor

Cofactor Comment Organism Structure
Fe-S center the small subunits contains a [2Fe-2S]-center Pseudomonas nitroreducens