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Literature summary for 1.2.99.7 extracted from

  • Correia, H.D.; Marangon, J.; Brondino, C.D.; Moura, J.J.; Romao, M.J.; Gonzalez, P.J.; Santos-Silva, T.
    Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: reactivity and molecular details of the enzyme-substrate and enzyme-product interaction (2015), J. Biol. Inorg. Chem., 20, 219-229.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-phenylacetaldehyde substrate inhibition at high concentrations Megalodesulfovibrio gigas
3-phenylpropanal substrate inhibition at high concentrations, suicide substrate Megalodesulfovibrio gigas
benzaldehyde substrate inhibition at high concentrations Megalodesulfovibrio gigas
cinnamaldehyde substrate inhibition at high concentrations Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
Salicylaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.008
-
cinnamaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.025
-
benzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.029
-
3-phenylpropanal pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.044
-
2,3-Dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.117
-
3-Hydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.27
-
2-phenylacetaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
3.457
-
2,5-dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas Q46509
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas 2,3-dihydroxybenzoate + reduced 2,6-dichlorophenol-indophenol
-
?
2,5-dihydroxybenzaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas 2,5-dihydroxybenzoate + reduced 2,6-dichlorophenol-indophenol
-
?
2-phenylacetaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas 2-phenylacetate + reduced 2,6-dichlorophenol-indophenol
-
?
3-hydroxybenzaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas 3-hydroxybenzoate + reduced 2,6-dichlorophenol-indophenol
-
?
3-phenylpropanal + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas 3-phenylpropanoate + reduced 2,6-dichlorophenol-indophenol
-
?
benzaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas benzoate + reduced 2,6-dichlorophenol-indophenol
-
?
cinnamaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas cinnamate + reduced 2,6-dichlorophenol-indophenol
-
?
additional information no substrates: hypoxanthine, xanthine, 1-methylxanthine, 3-methylxanthine, 1,3,7-trimethylxanthine, allopurinol, oxypurinol, isoquinoline, nicotinate, formamide Megalodesulfovibrio gigas ?
-
?
salicylaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
Megalodesulfovibrio gigas salicylate + reduced 2,6-dichlorophenol-indophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
Salicylaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.58
-
cinnamaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.87
-
2,3-Dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
2.1
-
3-phenylpropanal pH 7.6, 35┬░C Megalodesulfovibrio gigas
3.3
-
2,5-dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
3.6
-
3-Hydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
4.4
-
2-phenylacetaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
5.7
-
benzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
cinnamaldehyde Kis value, pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.6
-
3-phenylpropanal Kis value, pH 7.6, 35┬░C Megalodesulfovibrio gigas
0.8
-
benzaldehyde Kis value, pH 7.6, 35┬░C Megalodesulfovibrio gigas
4
-
2-phenylacetaldehyde Kis value, pH 7.6, 35┬░C Megalodesulfovibrio gigas

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
2,5-dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
16
-
2-phenylacetaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
20
-
2,3-Dihydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
31
-
3-Hydroxybenzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
72
-
3-phenylpropanal pH 7.6, 35┬░C Megalodesulfovibrio gigas
73
-
cinnamaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
228
-
benzaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas
275
-
Salicylaldehyde pH 7.6, 35┬░C Megalodesulfovibrio gigas