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Literature summary for 1.2.99.7 extracted from

  • Santos-Silva, T.; Ferroni, F.; Thapper, A.; Marangon, J.; Gonzalez, P.J.; Rizzi, A.C.; Moura, I.; Moura, J.J.; Romao, M.J.; Brondino, C.D.
    Kinetic, structural, and EPR studies reveal that aldehyde oxidoreductase from Desulfovibrio gigas does not need a sulfido ligand for catalysis and give evidence for a direct Mo-C interaction in a biological system (2009), J. Am. Chem. Soc., 131, 7990-7998.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 30% (v/v) 2-propanol, 0.2 M magnesium chloride, and 0.2 M HEPES buffer (pH 7.6) Megalodesulfovibrio gigas

Inhibitors

Inhibitors Comment Organism Structure
arsenite irreversible inhibitor, complete inhibition at 0.024 mM Megalodesulfovibrio gigas
cyanide competitive reversible inhibitor, 30.9% inhibition at 50 mM Megalodesulfovibrio gigas
ethylene glycol mixed-type reversible inhibitor, 21.9% inhibition at 1 mM Megalodesulfovibrio gigas
glycerol competitive reversible inhibitor, 52% inhibition at 1 mM Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
benzaldehyde in 50 mM Tris-HCl buffer (pH 7.6), at 37┬░C Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum contains molybdenum Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas Q46509
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
Megalodesulfovibrio gigas benzoate + reduced 2,6-dichlorophenol-indophenol
-
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Synonyms

Synonyms Comment Organism
aldehyde oxidoreductase
-
Megalodesulfovibrio gigas
AOR
-
Megalodesulfovibrio gigas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12
-
benzaldehyde in 50 mM Tris-HCl buffer (pH 7.6), at 37┬░C Megalodesulfovibrio gigas