BRENDA - Enzyme Database show
show all sequences of 1.2.99.7

Kinetic, structural, and EPR studies reveal that aldehyde oxidoreductase from Desulfovibrio gigas does not need a sulfido ligand for catalysis and give evidence for a direct Mo-C interaction in a biological system

Santos-Silva, T.; Ferroni, F.; Thapper, A.; Marangon, J.; Gonzalez, P.J.; Rizzi, A.C.; Moura, I.; Moura, J.J.; Romao, M.J.; Brondino, C.D.; J. Am. Chem. Soc. 131, 7990-7998 (2009)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (v/v) 2-propanol, 0.2 M magnesium chloride, and 0.2 M HEPES buffer (pH 7.6)
Desulfovibrio gigas
Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
irreversible inhibitor, complete inhibition at 0.024 mM
Desulfovibrio gigas
cyanide
competitive reversible inhibitor, 30.9% inhibition at 50 mM
Desulfovibrio gigas
ethylene glycol
mixed-type reversible inhibitor, 21.9% inhibition at 1 mM
Desulfovibrio gigas
glycerol
competitive reversible inhibitor, 52% inhibition at 1 mM
Desulfovibrio gigas
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0097
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Molybdenum
contains molybdenum
Desulfovibrio gigas
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Desulfovibrio gigas
Q46509
-
-
Purification (Commentary)
Commentary
Organism
-
Desulfovibrio gigas
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
712240
Desulfovibrio gigas
benzoate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.12
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (v/v) 2-propanol, 0.2 M magnesium chloride, and 0.2 M HEPES buffer (pH 7.6)
Desulfovibrio gigas
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
irreversible inhibitor, complete inhibition at 0.024 mM
Desulfovibrio gigas
cyanide
competitive reversible inhibitor, 30.9% inhibition at 50 mM
Desulfovibrio gigas
ethylene glycol
mixed-type reversible inhibitor, 21.9% inhibition at 1 mM
Desulfovibrio gigas
glycerol
competitive reversible inhibitor, 52% inhibition at 1 mM
Desulfovibrio gigas
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0097
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Molybdenum
contains molybdenum
Desulfovibrio gigas
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
712240
Desulfovibrio gigas
benzoate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.12
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Other publictions for EC 1.2.99.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740765
Correia
Aromatic aldehydes at the acti ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
219-229
2015
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4
8
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8
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4
8
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8
8
740766
Gomez
Isotropic exchange interaction ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
233-242
2015
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741287
Marangon
Kinetic and structural studies ...
Desulfovibrio gigas
PLoS ONE
8
e83234
2013
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1
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725548
Maia
Nitrite reduction by xanthine ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
16
443-460
2011
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712240
Santos-Silva
Kinetic, structural, and EPR s ...
Desulfovibrio gigas
J. Am. Chem. Soc.
131
7990-7998
2009
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1
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4
1
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1
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2
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1
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1
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1
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1
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655476
Correia dos Santos
Direct electrochemistry of the ...
Desulfovibrio gigas
Eur. J. Biochem.
271
1329-1338
2004
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644705
Uchida
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. KY 469
FEMS Microbiol. Lett.
229
31-36
2003
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656294
Rebelo
Structure refinement of the al ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
6
791-800
2001
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644707
Andrade
Aldehyde oxidoreductase activi ...
Desulfovibrio alaskensis, Desulfovibrio alaskensis NCIMB 1349, Desulfovibrio alaskensis NCIMB 13491
Eur. J. Biochem.
267
2054-2061
2000
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2
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1
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6
1
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6
1
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1
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654573
Caldeira
Analysis of the electron param ...
Desulfovibrio gigas
Biochemistry
39
2700-2707
2000
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657173
Huber
A structure-based catalytic me ...
Desulfovibrio gigas
Proc. Natl. Acad. Sci. USA
93
8846-8851
1996
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11936
Hensgens
Purification and characterizat ...
Desulfovibrio gigas
J. Bacteriol.
177
6195-6200
1995
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4
5
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3
3
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1
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15
1
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1
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4
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5
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3
3
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15
1
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1
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655399
Thoenes
Molecular cloning and sequence ...
Desulfovibrio gigas
Eur. J. Biochem.
220
901-910
1994
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654563
Barata
Aldehyde oxidoreductase activi ...
Desulfovibrio gigas
Biochemistry
32
11559-11568
1993
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4
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1
10
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4
1
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4
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1
10
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1
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655397
Romao
Subunit composition, crystalli ...
Desulfovibrio gigas
Eur. J. Biochem.
215
729-732
1993
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2
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654473
Bray
Information from EPR spectrosc ...
Desulfovibrio gigas
Biochem. J.
280
817-820
1991
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390386
Turner
The molybdenum iron-sulphur pr ...
Desulfovibrio gigas
Biochem. J.
243
755-761
1987
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