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show all sequences of 1.2.99.7

Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas

Hensgens, C.M.H.; Hagen, W.R.; Hansen, T.A.; J. Bacteriol. 177, 6195-6200 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
5 mM, complete inhibition
Desulfovibrio gigas
iodoacetate
5 mM, complete inhibition
Desulfovibrio gigas
KCN
5 mM, 64% inhibition
Desulfovibrio gigas
methanol
1 M, 62% inhibition
Desulfovibrio gigas
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
benzylviologen
30°C, pH 7.5
Desulfovibrio gigas
10.8
-
propionaldehyde
30°C, pH 7.5
Desulfovibrio gigas
12.5
-
acetaldehyde
30°C, pH 7.5
Desulfovibrio gigas
20
-
benzaldehyde
30°C, pH 7.5
Desulfovibrio gigas
30.8
-
furfural
30°C, pH 7.5
Desulfovibrio gigas
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
4.8 iron per subunit, probably a [Fe-4S]1+ cluster
Desulfovibrio gigas
K+
10 mM, 50% activation
Desulfovibrio gigas
Tungsten
0.68 tungsten per subunit
Desulfovibrio gigas
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
120000
-
gelfiltration, Sephacryl S-300
Desulfovibrio gigas
126000
-
gel filtration, Superose 12 HR
Desulfovibrio gigas
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Desulfovibrio gigas
-
-
-
Oxidation Stability
Oxidation Stability
Organism
exposure to air leads to inactivation
Desulfovibrio gigas
Purification (Commentary)
Commentary
Organism
Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010
Desulfovibrio gigas
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38.5
-
-
Desulfovibrio gigas
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + benzylviologen
natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively
11936
Desulfovibrio gigas
acetate + reduced benzylviologen
-
-
-
?
benzaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
benzaldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
butyraldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
butanoate + reduced benzylviologen
-
-
-
?
formaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
formate + reduced benzylviologen
-
-
-
?
furfural + H2O + benzylviologen
77% of activity with acetaldehyde
11936
Desulfovibrio gigas
furfurate + reduced benzylviologen
-
-
-
?
glutaraldehyde + H2O + benzylviologen
20% of activity with acetaldehyde
11936
Desulfovibrio gigas
glutarate + reduced benzylviologen
-
-
-
?
glycoaldehyde + H2O + benzylviologen
23% of activity with acetaldehyde
11936
Desulfovibrio gigas
glycolate + reduced benzylviologen
-
-
-
?
hexanal + H2O + benzylviologen
6% of activity with acetaldehyde
11936
Desulfovibrio gigas
hexanoate + reduced benzylviologen
-
-
-
?
octanal + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
octanoate + reduced benzylviologen
-
-
-
?
p-anisaldehyde + H2O + benzylviologen
5% of activity with acetaldehyde
11936
Desulfovibrio gigas
p-anisidic acid + reduced benzylviologen
-
-
-
?
pentanal + H2O + benzylviologen
9% of activity with acetaldehyde
11936
Desulfovibrio gigas
pentanoate + reduced benzylviologen
-
-
-
?
phenylacetaldehyde + H2O + benzylviologen
31% of activity with acetaldehyde
11936
Desulfovibrio gigas
phenylacetate + reduced benzylviologen
-
-
-
?
propionaldehyde + H2O + benzylviologen
117% of activity with acetaldehyde
11936
Desulfovibrio gigas
propionate + reduced benzylviologen
-
-
-
?
salicylaldehyde + H2O + benzylviologen
14% of activity with acetaldehyde
11936
Desulfovibrio gigas
salicylate + reduced benzylviologen
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
65
-
Desulfovibrio gigas
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
broad optimum
Desulfovibrio gigas
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
5 mM, complete inhibition
Desulfovibrio gigas
iodoacetate
5 mM, complete inhibition
Desulfovibrio gigas
KCN
5 mM, 64% inhibition
Desulfovibrio gigas
methanol
1 M, 62% inhibition
Desulfovibrio gigas
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
benzylviologen
30°C, pH 7.5
Desulfovibrio gigas
10.8
-
propionaldehyde
30°C, pH 7.5
Desulfovibrio gigas
12.5
-
acetaldehyde
30°C, pH 7.5
Desulfovibrio gigas
20
-
benzaldehyde
30°C, pH 7.5
Desulfovibrio gigas
30.8
-
furfural
30°C, pH 7.5
Desulfovibrio gigas
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
4.8 iron per subunit, probably a [Fe-4S]1+ cluster
Desulfovibrio gigas
K+
10 mM, 50% activation
Desulfovibrio gigas
Tungsten
0.68 tungsten per subunit
Desulfovibrio gigas
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
2 * 62000, SDS-PAGE
Desulfovibrio gigas
120000
-
gelfiltration, Sephacryl S-300
Desulfovibrio gigas
126000
-
gel filtration, Superose 12 HR
Desulfovibrio gigas
Oxidation Stability (protein specific)
Oxidation Stability
Organism
exposure to air leads to inactivation
Desulfovibrio gigas
Purification (Commentary) (protein specific)
Commentary
Organism
Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010
Desulfovibrio gigas
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38.5
-
-
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + benzylviologen
natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively
11936
Desulfovibrio gigas
acetate + reduced benzylviologen
-
-
-
?
benzaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
benzaldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
benzoate + reduced benzylviologen
-
-
-
?
butyraldehyde + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
butanoate + reduced benzylviologen
-
-
-
?
formaldehyde + H2O + benzylviologen
16% of activity with acetaldehyde
11936
Desulfovibrio gigas
formate + reduced benzylviologen
-
-
-
?
furfural + H2O + benzylviologen
77% of activity with acetaldehyde
11936
Desulfovibrio gigas
furfurate + reduced benzylviologen
-
-
-
?
glutaraldehyde + H2O + benzylviologen
20% of activity with acetaldehyde
11936
Desulfovibrio gigas
glutarate + reduced benzylviologen
-
-
-
?
glycoaldehyde + H2O + benzylviologen
23% of activity with acetaldehyde
11936
Desulfovibrio gigas
glycolate + reduced benzylviologen
-
-
-
?
hexanal + H2O + benzylviologen
6% of activity with acetaldehyde
11936
Desulfovibrio gigas
hexanoate + reduced benzylviologen
-
-
-
?
octanal + H2O + benzylviologen
93% of activity with acetaldehyde
11936
Desulfovibrio gigas
octanoate + reduced benzylviologen
-
-
-
?
p-anisaldehyde + H2O + benzylviologen
5% of activity with acetaldehyde
11936
Desulfovibrio gigas
p-anisidic acid + reduced benzylviologen
-
-
-
?
pentanal + H2O + benzylviologen
9% of activity with acetaldehyde
11936
Desulfovibrio gigas
pentanoate + reduced benzylviologen
-
-
-
?
phenylacetaldehyde + H2O + benzylviologen
31% of activity with acetaldehyde
11936
Desulfovibrio gigas
phenylacetate + reduced benzylviologen
-
-
-
?
propionaldehyde + H2O + benzylviologen
117% of activity with acetaldehyde
11936
Desulfovibrio gigas
propionate + reduced benzylviologen
-
-
-
?
salicylaldehyde + H2O + benzylviologen
14% of activity with acetaldehyde
11936
Desulfovibrio gigas
salicylate + reduced benzylviologen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 62000, SDS-PAGE
Desulfovibrio gigas
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
65
-
Desulfovibrio gigas
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
broad optimum
Desulfovibrio gigas
Other publictions for EC 1.2.99.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740765
Correia
Aromatic aldehydes at the acti ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
219-229
2015
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740766
Gomez
Isotropic exchange interaction ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
233-242
2015
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741287
Marangon
Kinetic and structural studies ...
Desulfovibrio gigas
PLoS ONE
8
e83234
2013
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725548
Maia
Nitrite reduction by xanthine ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
16
443-460
2011
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712240
Santos-Silva
Kinetic, structural, and EPR s ...
Desulfovibrio gigas
J. Am. Chem. Soc.
131
7990-7998
2009
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655476
Correia dos Santos
Direct electrochemistry of the ...
Desulfovibrio gigas
Eur. J. Biochem.
271
1329-1338
2004
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644705
Uchida
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. KY 469
FEMS Microbiol. Lett.
229
31-36
2003
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656294
Rebelo
Structure refinement of the al ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
6
791-800
2001
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644707
Andrade
Aldehyde oxidoreductase activi ...
Desulfovibrio alaskensis, Desulfovibrio alaskensis NCIMB 1349, Desulfovibrio alaskensis NCIMB 13491
Eur. J. Biochem.
267
2054-2061
2000
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654573
Caldeira
Analysis of the electron param ...
Desulfovibrio gigas
Biochemistry
39
2700-2707
2000
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657173
Huber
A structure-based catalytic me ...
Desulfovibrio gigas
Proc. Natl. Acad. Sci. USA
93
8846-8851
1996
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11936
Hensgens
Purification and characterizat ...
Desulfovibrio gigas
J. Bacteriol.
177
6195-6200
1995
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4
5
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3
3
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1
1
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15
1
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655399
Thoenes
Molecular cloning and sequence ...
Desulfovibrio gigas
Eur. J. Biochem.
220
901-910
1994
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654563
Barata
Aldehyde oxidoreductase activi ...
Desulfovibrio gigas
Biochemistry
32
11559-11568
1993
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4
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2
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1
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1
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1
1
10
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4
1
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4
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1
10
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1
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655397
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Subunit composition, crystalli ...
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Information from EPR spectrosc ...
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390386
Turner
The molybdenum iron-sulphur pr ...
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