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Literature summary for 1.2.99.7 extracted from

  • Hensgens, C.M.H.; Hagen, W.R.; Hansen, T.A.
    Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas (1995), J. Bacteriol., 177, 6195-6200.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
arsenite 5 mM, complete inhibition Megalodesulfovibrio gigas
iodoacetate 5 mM, complete inhibition Megalodesulfovibrio gigas
KCN 5 mM, 64% inhibition Megalodesulfovibrio gigas
methanol 1 M, 62% inhibition Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
benzylviologen 30┬░C, pH 7.5 Megalodesulfovibrio gigas
10.8
-
propionaldehyde 30┬░C, pH 7.5 Megalodesulfovibrio gigas
12.5
-
acetaldehyde 30┬░C, pH 7.5 Megalodesulfovibrio gigas
20
-
benzaldehyde 30┬░C, pH 7.5 Megalodesulfovibrio gigas
30.8
-
furfural 30┬░C, pH 7.5 Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 4.8 iron per subunit, probably a [Fe-4S]1+ cluster Megalodesulfovibrio gigas
K+ 10 mM, 50% activation Megalodesulfovibrio gigas
Tungsten 0.68 tungsten per subunit Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
2 * 62000, SDS-PAGE Megalodesulfovibrio gigas
120000
-
gelfiltration, Sephacryl S-300 Megalodesulfovibrio gigas
126000
-
gel filtration, Superose 12 HR Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
-
-

Oxidation Stability

Oxidation Stability Organism
exposure to air leads to inactivation Megalodesulfovibrio gigas

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
38.5
-
-
Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + benzylviologen natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively Megalodesulfovibrio gigas acetate + reduced benzylviologen
-
?
benzaldehyde + H2O + benzylviologen 16% of activity with acetaldehyde Megalodesulfovibrio gigas benzoate + reduced benzylviologen
-
?
benzaldehyde + H2O + benzylviologen 93% of activity with acetaldehyde Megalodesulfovibrio gigas benzoate + reduced benzylviologen
-
?
butyraldehyde + H2O + benzylviologen 93% of activity with acetaldehyde Megalodesulfovibrio gigas butanoate + reduced benzylviologen
-
?
formaldehyde + H2O + benzylviologen 16% of activity with acetaldehyde Megalodesulfovibrio gigas formate + reduced benzylviologen
-
?
furfural + H2O + benzylviologen 77% of activity with acetaldehyde Megalodesulfovibrio gigas furfurate + reduced benzylviologen
-
?
glutaraldehyde + H2O + benzylviologen 20% of activity with acetaldehyde Megalodesulfovibrio gigas glutarate + reduced benzylviologen
-
?
glycoaldehyde + H2O + benzylviologen 23% of activity with acetaldehyde Megalodesulfovibrio gigas glycolate + reduced benzylviologen
-
?
hexanal + H2O + benzylviologen 6% of activity with acetaldehyde Megalodesulfovibrio gigas hexanoate + reduced benzylviologen
-
?
octanal + H2O + benzylviologen 93% of activity with acetaldehyde Megalodesulfovibrio gigas octanoate + reduced benzylviologen
-
?
p-anisaldehyde + H2O + benzylviologen 5% of activity with acetaldehyde Megalodesulfovibrio gigas p-anisic acid + reduced benzylviologen
-
?
pentanal + H2O + benzylviologen 9% of activity with acetaldehyde Megalodesulfovibrio gigas pentanoate + reduced benzylviologen
-
?
phenylacetaldehyde + H2O + benzylviologen 31% of activity with acetaldehyde Megalodesulfovibrio gigas phenylacetate + reduced benzylviologen
-
?
propionaldehyde + H2O + benzylviologen 117% of activity with acetaldehyde Megalodesulfovibrio gigas propionate + reduced benzylviologen
-
?
salicylaldehyde + H2O + benzylviologen 14% of activity with acetaldehyde Megalodesulfovibrio gigas salicylate + reduced benzylviologen
-
?

Subunits

Subunits Comment Organism
dimer 2 * 62000, SDS-PAGE Megalodesulfovibrio gigas

Synonyms

Synonyms Comment Organism
BV-AIDH
-
Megalodesulfovibrio gigas

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 65
-
Megalodesulfovibrio gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
broad optimum Megalodesulfovibrio gigas