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Literature summary for 1.2.99.6 extracted from

  • Strobl, G.; Feicht, R.; White, H.; Lottspeich, F.; Simon, H.
    The tungsten-containing aldehyde oxidoreductase from Clostridium thermoaceticum and its complex with a viologen-accepting NADPH oxidoreductase (1992), Biol. Chem. Hoppe-Seyler, 373, 123-132.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the heptameric enzyme form contains 75-90 mol of iron per mol of enzyme Moorella thermoacetica
Iron the dimeric enzyme form contains 25-33 mol of iron per mol of enzyme Moorella thermoacetica
Tungsten the heptameric enzyme form contains 3.4 mol of tungsten per mol of enzyme Moorella thermoacetica
Tungsten the dimeric form contains 0.8 mol of tungsten per mol of enzyme Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
14000
-
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
43000
-
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
64000
-
1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
64000
-
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
86000
-
dimeric form: alpha,beta, gel filtration Moorella thermoacetica
300000
-
heptameric form: alpha3,beta3,gamma, gel filtration Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in contast to the dimeric enzyme form the heptameric enzyme form also catalyzes the reversible reduction of the oxidized carbamoyl methyl viologen with NADPH Moorella thermoacetica ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica
heptamer 3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
FAD the heptameric enzyme form contains 1.7 mol of FAD per mol of enzyme, seems to be a component of the gamma-subunit Moorella thermoacetica
pterin the dimeric enzyme form contains 0.8-1.1 mol of pterine per mol of enzyme Moorella thermoacetica
pterin the heptameric enzyme form contains 2.5 mol of pterine per mol of enzyme Moorella thermoacetica