Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the heptameric enzyme form contains 75-90 mol of iron per mol of enzyme | Moorella thermoacetica | |
Iron | the dimeric enzyme form contains 25-33 mol of iron per mol of enzyme | Moorella thermoacetica | |
Tungsten | the heptameric enzyme form contains 3.4 mol of tungsten per mol of enzyme | Moorella thermoacetica | |
Tungsten | the dimeric form contains 0.8 mol of tungsten per mol of enzyme | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
14000 | - |
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
43000 | - |
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
64000 | - |
1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
64000 | - |
3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
86000 | - |
dimeric form: alpha,beta, gel filtration | Moorella thermoacetica |
300000 | - |
heptameric form: alpha3,beta3,gamma, gel filtration | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in contast to the dimeric enzyme form the heptameric enzyme form also catalyzes the reversible reduction of the oxidized carbamoyl methyl viologen with NADPH | Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 14000, beta, + 1 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
heptamer | 3 * 14000, + 1 * 43000, gamma, + 3 * 64000, alpha, enzyme exists in a dimeric and a heptameric form, SDS-PAGE | Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the heptameric enzyme form contains 1.7 mol of FAD per mol of enzyme, seems to be a component of the gamma-subunit | Moorella thermoacetica | |
pterin | the dimeric enzyme form contains 0.8-1.1 mol of pterine per mol of enzyme | Moorella thermoacetica | |
pterin | the heptameric enzyme form contains 2.5 mol of pterine per mol of enzyme | Moorella thermoacetica |