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Literature summary for 1.2.99.6 extracted from

  • Heider, J.; Ma, K.; Adams, M.W.W.
    Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1 (1995), J. Bacteriol., 177, 4757-4764.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.99.6

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl
-
 Thermococcus sp.
acetaldehyde 5 mM, 50% inhibition Thermococcus sp.
AsO2-
-
 Thermococcus sp.
CN- relatively insensitive, 10% inhibition after 1 with 8 mM Thermococcus sp.
iodoacetate
-
 Thermococcus sp.
Isovaleraldehyde 4 mM, 50% inhibition Thermococcus sp.
PCMB
-
 Thermococcus sp.
phenylacetaldehyde 7 mM, 50% inhibition Thermococcus sp.
ZnCl2
-
 Thermococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ES-1 ferredoxin with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
0.016
-
 acetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.028
-
 Isovaleraldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.05
-
 Indoleacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.057
-
 benzaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.065
-
 Salicylaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.076
-
 phenylacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.13
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
0.136
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.15
-
 propanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
0.169
-
 benzyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
0.196
-
 glyceraldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
1.422
-
 methanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.8
-
 acetate with methylviologen as cosubstrate Thermococcus sp.
2.9
-
 methyl viologen with crotonaldehyde as cosubstrate Thermococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme contains 0.7 gatom of Ca per mol of enzyme Thermococcus sp.
Iron enzyme contains 3.6 gatom Fe per mol of enzyme Thermococcus sp.
Iron contains a single [4Fe-4S]+cluster with an S = 3/2 ground state Thermococcus sp.
Mg2+ enzyme contains 1.1 gatom of Mg per mol of enzyme Thermococcus sp.
Tungsten enzyme contains 0.9 gatom of tungsten per mol of enzyme Thermococcus sp.
Zn2+ the enzyme contains 0.2 gatom of Zn per mol of enzyme Thermococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
105000
-
 gel filtration Thermococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermococcus sp. the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?
additional information Thermococcus sp. ES-1 the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?
additional information Thermococcus sp. ES1 the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Thermococcus sp. ES-1
-
-
-

Oxidation Stability

Oxidation Stability Organism
extremely sensitive to inactivation by O2. The extent of inactivation is greatly dependent on the temperature of incubation Thermococcus sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ethanol-grown cell
-
 Thermococcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
221
-
-
 Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetaldehyde + benzyl viologen r Thermococcus sp. acetate + reduced benzyl viologen
-
 ?
Acetaldehyde + benzyl viologen r Thermococcus sp. ES-1 acetate + reduced benzyl viologen
-
 ?
Acetaldehyde + benzyl viologen r Thermococcus sp. ES1 acetate + reduced benzyl viologen
-
 ?
Benzaldehyde + benzyl viologen
-
 Thermococcus sp. Benzoate + reduced benzyl viologen
-
 ?
Benzaldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Benzoate + reduced benzyl viologen
-
 ?
Benzaldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Benzoate + reduced benzyl viologen
-
 ?
Crotonaldehyde + benzyl viologen at less than 1% of the activity with benzyl viologen Thermococcus sp. E-2-Butenoate + reduced benzyl viologen
-
 ?
Crotonaldehyde + ES-1 ferredoxin
-
 Thermococcus sp. Crotonate + reduced ES-1 ferredoxin
-
 ?
Crotonaldehyde + methyl viologen
-
 Thermococcus sp. Crotonate + reduced methyl viologen
-
 ?
Glyceraldehyde + benzyl viologen
-
 Thermococcus sp. Glycerate + reduced benzyl viologen
-
 ?
Indoleacetaldehyde + benzyl viologen
-
 Thermococcus sp. Indoleacetate + reduced benzyl viologen
-
 ?
Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. Isovalerate + reduced benzyl viologen
-
 ?
Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Isovalerate + reduced benzyl viologen
-
 ?
Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Isovalerate + reduced benzyl viologen
-
 ?
Methanal + benzyl viologen
-
 Thermococcus sp. Formate + reduced benzyl viologen
-
 ?
additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ?
-
 ?
additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ES-1 ?
-
 ?
additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ES1 ?
-
 ?
Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. Phenylacetate + reduced benzyl viologen
-
 ?
Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Phenylacetate + reduced benzyl viologen
-
 ?
Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Phenylacetate + reduced benzyl viologen
-
 ?
Propanal + benzyl viologen
-
 Thermococcus sp. Propanoate + reduced benzyl viologen
-
 ?
Salicylaldehyde + benzyl viologen
-
 Thermococcus sp. Salicylate + reduced benzyl viologen
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 67000, SDS-PAGE Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 enzyme concentration 2.0 mg/ml, 50% loss of activity after 5 min Thermococcus sp.
85
-
 enzyme concentration 2.0 mg/ml, 50% loss of activity after 1 min Thermococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 acetaldehyde with methyl viologen as cosubstrate Thermococcus sp.
10.5
-
 glyceraldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
13
-
 Salicylaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
22
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
55
-
 indolecetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
62
-
 methyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
250
-
 benzyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
269
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
270
-
 ES-1 ferredoxin with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
272
-
 Isovaleraldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
343
-
 acetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
720
-
 benzaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
950
-
 methanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
960
-
 phenylacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1100
-
 propanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 around, at 80 C, with benzyl viologen and crotonaldehyde as substrate Thermococcus sp.

Cofactor

Cofactor Comment Organism Structure
molybdopterin enzyme contains molybdopterin Thermococcus sp.