BRENDA - Enzyme Database
show all sequences of 1.2.99.10

Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde

Tao, L.; Schenzle, A.; Odom, J.; Cheng, Q.; Appl. Environ. Microbiol. 71, 3294-3301 (2005) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Methylomonas sp. 16a
Organism
Organism
UniProt
Commentary
Textmining
Methylomonas sp. 16a
Q4VKU9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4,4'-diapolycopene + H2O + acceptor
-
744091
Methylomonas sp. 16a
4,4'-diapolycopene-4,4'-dial + reduced acceptor
-
-
-
?
4,4'-diaponeurosporene + H2O + acceptor
high activity
744091
Methylomonas sp. 16a
4,4'-diaponeurosporen-4-al + reduced acceptor
-
-
-
?
additional information
the enzyme has moderate to low activity on neurosporene and lycopene and no activity on beta-carotene or zeta-carotene
744091
Methylomonas sp. 16a
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CrtN2
-
Methylomonas sp. 16a
CrtNb
-
Methylomonas sp. 16a
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Methylomonas sp. 16a
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4,4'-diapolycopene + H2O + acceptor
-
744091
Methylomonas sp. 16a
4,4'-diapolycopene-4,4'-dial + reduced acceptor
-
-
-
?
4,4'-diaponeurosporene + H2O + acceptor
high activity
744091
Methylomonas sp. 16a
4,4'-diaponeurosporen-4-al + reduced acceptor
-
-
-
?
additional information
the enzyme has moderate to low activity on neurosporene and lycopene and no activity on beta-carotene or zeta-carotene
744091
Methylomonas sp. 16a
?
-
-
-
?
Other publictions for EC 1.2.99.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745725
Steiger
Annotation and functional ass ...
Metabacillus indicus, Cytobacillus firmus
Microbiology
161
194-202
2015
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2
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4
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7
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4
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4
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713818
Tao
Novel carotenoid oxidase invol ...
Methylomonas sp.
Appl. Environ. Microbiol.
71
3294-3301
2005
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1
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1
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1
1
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744091
Tao
Novel carotenoid oxidase invo ...
Methylomonas sp. 16a
Appl. Environ. Microbiol.
71
3294-3301
2005
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-
1
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1
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3
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