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Literature summary for 1.2.98.1 extracted from

  • Imoto, S.; Yonemitsu, H.; Totsui, N.; Kishimoto, N.
    Purification and characterization of formaldehyde dismutases of Methylobacterium sp. FD1 (2020), Biosci. Biotechnol. Biochem., 84, 1444-1450 .
    View publication on PubMed
Search for more literature for 1.2.98.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylobacterium sp. FD1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4 10 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
620
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
870
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1.7 mol per mol of enzyme Methylobacterium sp. FD1
Zn2+ 1.2 mol per mol of enzyme Methylobacterium sp. FD1
Zn2+ 0.7 mol per mol of enzyme Methylobacterium sp. FD1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
181000
-
 gel filtration Methylobacterium sp. FD1
184000
-
 gel filtration Methylobacterium sp. FD1
188000
-
 gel filtration Methylobacterium sp. FD1

Organism

Organism UniProt Comment Textmining
Methylobacterium sp. FD1 A0A224AQI3 isoform Fdm1
-
Methylobacterium sp. FD1 A0A6F8QRG1 isoform Fdm2
-
Methylobacterium sp. FD1 A0A6F8QRG1 isoform Fdm3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, by immobilized metal affinity chromatography Methylobacterium sp. FD1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 butanal + H2O
-
 Methylobacterium sp. FD1 butanoate + 1-butanol
-
 ?
2 formaldehyde + H2O
-
 Methylobacterium sp. FD1 formate + methanol
-
 ?
2 propanal + H2O
-
 Methylobacterium sp. FD1 propanoate + 1-propanol
-
 ?
additional information enzyme additionally displays methanol:p-nitroso-N,N-dimethylaniline oxidoreductase activity, reaction of EC 1.1.99.36 Methylobacterium sp. FD1 ?
-
-

Subunits

Subunits Comment Organism
tetramer 4 * 43600, SDS-PAGE Methylobacterium sp. FD1
tetramer 4 * 43700, SDS-PAGE Methylobacterium sp. FD1
tetramer 4 * 44600, SDS-PAGE Methylobacterium sp. FD1

Synonyms

Synonyms Comment Organism
FDM
-
 Methylobacterium sp. FD1
Fdm1
-
 Methylobacterium sp. FD1
Fdm2
-
 Methylobacterium sp. FD1
Fdm3
-
 Methylobacterium sp. FD1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Methylobacterium sp. FD1
30
-
-
 Methylobacterium sp. FD1
35
-
-
 Methylobacterium sp. FD1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3000
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
4300
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
7500
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Methylobacterium sp. FD1
6.5
-
-
 Methylobacterium sp. FD1

Cofactor

Cofactor Comment Organism Structure
NAD+ bound to enzyme Methylobacterium sp. FD1
NADH bound to enzyme Methylobacterium sp. FD1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.6
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
6.9
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1
8.6
-
 formaldehyde pH 7, 30°C Methylobacterium sp. FD1