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Literature summary for 1.2.98.1 extracted from

  • Kato, N.; Kobayashi, H.; Shimano, M.; Sakazawa, C.
    Properties of formaldehyde dismutation catalyzing enzyme of Pseudomonas putida F61 (1984), Agric. Biol. Chem., 48, 2017-2023.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Pseudomonas putida
hydroxylamine
-
Pseudomonas putida
NaBH4
-
Pseudomonas putida
Semicarbazide
-
Pseudomonas putida
thiosulfate
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Pseudomonas putida
220000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
2 forms
-
Pseudomonas putida
-
F61
-
Pseudomonas putida F61
-
F61
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91.1
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + H2O dismutation Pseudomonas putida formate + methanol
-
?
formaldehyde + H2O dismutation Pseudomonas putida F61 formate + methanol
-
?
formaldehyde + H2O + acrolein coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + allyl alcohol
-
?
formaldehyde + H2O + acrolein cross-dismutation Pseudomonas putida formate + allyl alcohol
-
?
formaldehyde + H2O + acrolein in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + allyl alcohol
-
?
formaldehyde + H2O + acrolein coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida F61 formate + allyl alcohol
-
?
formaldehyde + H2O + butyraldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + butanol
-
?
formaldehyde + H2O + butyraldehyde cross-dismutation Pseudomonas putida formate + butanol
-
?
formaldehyde + H2O + butyraldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + butanol
-
?
formaldehyde + H2O + crotonaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + crotonyl alcohol
-
?
formaldehyde + H2O + crotonaldehyde cross-dismutation Pseudomonas putida formate + crotonyl alcohol
-
?
formaldehyde + H2O + crotonaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + crotonyl alcohol
-
?
formaldehyde + H2O + isobutyraldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + isobutanol
-
?
formaldehyde + H2O + isobutyraldehyde cross-dismutation Pseudomonas putida formate + isobutanol
-
?
formaldehyde + H2O + isobutyraldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + isobutanol
-
?
formaldehyde + H2O + propionaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + propanol
-
?
formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida formate + propanol
-
?
formaldehyde + H2O + propionaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + propanol
-
?
formaldehyde + H2O + propionaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida F61 formate + propanol
-
?
formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida F61 formate + propanol
-
?
formaldehyde + H2O + propionaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida F61 formate + propanol
-
?
n-butanol + H2O + isobutyraldehyde in general: RCH2OH + R'CHO = RCHO + R'CH2OH, aliphatic alcohols with longer carbon chains than C-5, secondary alcohols, diols and aromatic alcohols and the corresponding aldehydes are no substrates Pseudomonas putida butyraldehyde + isobutanol
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
Hg2+ noncompetitive inhibition Pseudomonas putida
0.52
-
Semicarbazide noncompetitive inhibition Pseudomonas putida