Literature summary for 1.2.7.B2 extracted from

Roy, R.; Mukund, S.; Schut, G.J.; Dunn, D.M.; Weiss, R.; Adams, M.W.
Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family (1999), J. Bacteriol., 181, 1171-1180.

Search for more literature for 1.2.7.B2

Activating Compound

Activating Compound	Comment	Organism	Structure
Sulfide	activated up to fivefold by treatment with sulfide under reducing conditions	Pyrococcus furiosus

Inhibitors

Inhibitors	Comment	Organism	Structure
glycerol	60% v/v, 6 h, 60% loss of activity	Pyrococcus furiosus
Glyoxal	above 1 mM	Pyrococcus furiosus
additional information	the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents	Pyrococcus furiosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	oxidized ferredoxin	80°C, pH 8.4	Pyrococcus furiosus
0.8	-	glutaric dialdehyde	80°C, pH 8.4	Pyrococcus furiosus
8	-	Succinic semialdehyde	80°C, pH 8.4	Pyrococcus furiosus
15	-	phenylpropionaldehyde	80°C, pH 8.4	Pyrococcus furiosus
25	-	formaldehyde	80°C, pH 8.4	Pyrococcus furiosus
25	-	Indole-3-acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
60	-	acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
62	-	propionaldehyde	80°C, pH 8.4	Pyrococcus furiosus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Calcium	contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit	Pyrococcus furiosus
Iron	contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit	Pyrococcus furiosus
Tungsten	tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68700	-	4 * 68700, calculated from sequence	Pyrococcus furiosus
275000	-	gel filtration	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	O93738	-	-

Oxidation Stability

Oxidation Stability	Organism
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions	Pyrococcus furiosus

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
58	-	pH 8.4, 90°C	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetaldehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	acetate + reduced benzyl viologen + H+	-	?
formaldehyde + H2O + 2 oxidized ferredoxin	formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM)	Pyrococcus furiosus	formate + 2 reduced ferredoxin + 2 H+	-	?
formaldehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	formate + reduced benzyl viologen + H+	-	?
glutaric dialdehyde + H2O + oxidized benzyl viologen	best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates	Pyrococcus furiosus	? + reduced benzyl viologen + H+	-	?
indole-3-acetaldehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	indole-3-acetate + reduced benzyl viologen + H+	-	?
additional information	low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate	Pyrococcus furiosus	?	-	?
phenylpropionaldehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	phenylpropionate + reduced benzyl viologen + H+	-	?
propionaldehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	propionate + reduced benzyl viologen + H+	-	?
succinic semialdehyde + H2O + oxidized benzyl viologen	-	Pyrococcus furiosus	succinate + reduced benzyl viologen + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 68700, calculated from sequence	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
FOR	-	Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase	-	Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	8 h, 50% loss of activity	Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2300	-	Indole-3-acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
5700	-	Succinic semialdehyde	80°C, pH 8.4	Pyrococcus furiosus
11000	-	propionaldehyde	80°C, pH 8.4	Pyrococcus furiosus
25000	-	phenylpropionaldehyde	80°C, pH 8.4	Pyrococcus furiosus
42000	-	glutaric dialdehyde	80°C, pH 8.4	Pyrococcus furiosus
44000	-	acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
71000	-	formaldehyde	80°C, pH 8.4	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C	Pyrococcus furiosus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10	the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C	Pyrococcus furiosus

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the enzyme contains a pterin cofactor	Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
90	-	Indole-3-acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
170	-	propionaldehyde	80°C, pH 8.4	Pyrococcus furiosus
710	-	Succinic semialdehyde	80°C, pH 8.4	Pyrococcus furiosus
730	-	acetaldehyde	80°C, pH 8.4	Pyrococcus furiosus
1700	-	phenylpropionaldehyde	80°C, pH 8.4	Pyrococcus furiosus
3000	-	formaldehyde	80°C, pH 8.4	Pyrococcus furiosus
60000	-	glutaric dialdehyde	80°C, pH 8.4	Pyrococcus furiosus

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Release 2023.1 (January 2023)