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Literature summary for 1.2.7.7 extracted from
- Isobutanol production by autotrophic acetogenic bacteria (2021), Front. Bioeng. Biotechnol., 9, 657253 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | expression of a isobutanol synthesis pathway using ketoisovalerate ferredoxin oxidoreductase (Kor) from Clostridium thermocellum and bifunctional aldehyde/alcohol dehydrogenase (AdhE2) from Clostidium acetobutylicum. Only in recombinant Acetobacterium woodii strains, traces of isobutanol can be detected. Additional feeding of ketoisovalerate increases isobutanol production to 2.9 mM under heterotrophic conditions using isoform Kor3 and to 1.8 mM under autotrophic conditions using isoform Kor2. In Clostidium ljungdahlii, isobutanol can only be detected upon additional ketoisovalerate feeding under autotrophic conditions. Isoform Kor3 is the best suited gene cluster | Acetivibrio thermocellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
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| Acetivibrio thermocellus DSM 1313 | - |
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Release 2023.1 (January 2023)
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