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Literature summary for 1.2.7.7 extracted from

  • Tersteegen, A.; Linder, D.; Thauer, R.K.; Hedderich, R.
    Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum (1997), Eur. J. Biochem., 244, 862-868.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
CoA pH 8.0, 65°C, anaerobic Methanothermobacter thermautotrophicus
1.3
-
2-ketoisovalerate pH 8.0, 65°C, anaerobic Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
37000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
55000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus
-
?
-
?
additional information Thermococcus litoralis
-
?
-
?
additional information Methanothermobacter thermautotrophicus enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium ?
-
?
additional information Methanothermobacter thermautotrophicus Marburg / DSM 2133 enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium ?
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
strain DSM 2133
-
Pyrococcus furiosus
-
-
-
Thermococcus litoralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions Methanothermobacter thermautotrophicus

Reaction

Reaction Comment Organism Reaction ID
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Methanothermobacter thermautotrophicus
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.8
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-ketobutyrate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-ketobutyrate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus Marburg / DSM 2133 propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-ketoisohexanoate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus isopentanoyl-CoA + CO2 + reduced benzyl viologen
-
?
2-ketoisovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
?
2-ketoisovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
?
2-ketovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus butanoyl-CoA + CO2 + reduced benzyl viologen
-
?
additional information
-
Pyrococcus furiosus ?
-
?
additional information
-
Thermococcus litoralis ?
-
?
additional information no substrate are 2-ketoglutarate and pyruvate Methanothermobacter thermautotrophicus ?
-
?
additional information enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium Methanothermobacter thermautotrophicus ?
-
?
additional information no substrate are 2-ketoglutarate and pyruvate Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?
additional information enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?
phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
phenylpyruvate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
phenylpyruvate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus Marburg / DSM 2133 phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?

Subunits

Subunits Comment Organism
? x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
? comparison of N-terminal amino acid sequences Methanothermobacter thermautotrophicus
? comparison of N-terminal amino acid sequences Pyrococcus furiosus
? comparison of N-terminal amino acid sequences Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
at pH 8.0 Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
at 65°C Methanothermobacter thermautotrophicus