Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is induced during glycolysis | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme contains iron-sulfur cluster | Pyrococcus furiosus | |
Tungsten | single tungsten environment is afforded for the reaction | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Pyrococcus furiosus |
64000 | - |
1 * 64000, SDS-PAGE | Pyrococcus furiosus |
74000 | - |
sequence determination | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | Pyrococcus furiosus | enzyme is a site for glycolytic regulation | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U3K2 | - |
- |
Pyrococcus furiosus | Q8U3K2 | hyperthermophilic archaeon | - |
Purification (Comment) | Organism |
---|---|
35fold, about 95% purity | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin = 3-phospho-D-glycerate + 2 H+ + reduced ferredoxin | this enzyme is thought to function in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase in the novel Embden-Meyerhoff-type glycolytic pathway found in Pyrococcus furiosus. It is specific for glyceraldehyde-3-phosphate | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
purified enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen | artificial electron acceptor | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | enzyme is a site for glycolytic regulation | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde-3-phosphate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | dependent on | Pyrococcus furiosus |