Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | activation of formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Sulfide | 4-5fold increase in activity at room temperature and pH 8.0 at 20 mM, in presence of 20 mM dithionite | Pyrococcus furiosus | |
Tungsten | stimulates cell growth | Pyrococcus furiosus | |
Tungsten | stimulates cell growth | Thermococcus litoralis |
Crystallization (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
acetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.028 | - |
isovalerylaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.05 | - |
Indoleacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.057 | - |
benzaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.076 | - |
phenylacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.14 | - |
crotonaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.15 | - |
propionaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.8 | - |
glutaric dialdehyde | pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor | Pyrococcus furiosus | |
1.42 | - |
formaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
8 | - |
Succinic semialdehyde | pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor | Pyrococcus furiosus | |
15 | - |
phenylpropionaldehyde | pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor | Pyrococcus furiosus | |
25 | - |
formaldehyde | formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor | Pyrococcus furiosus | |
25 | - |
Indoleacetaldehyde | pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor | Pyrococcus furiosus | |
60 | - |
acetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Pyrococcus furiosus | |
60 | - |
propionaldehyde | pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 Ca2+ per subunit, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Pyrococcus furiosus | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Thermococcus sp. | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Thermococcus litoralis | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Pyrococcus endeavori | |
Fe2+ | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus furiosus | |
Fe2+ | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus sp. | |
Fe2+ | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus litoralis | |
Fe2+ | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus endeavori | |
Fe2+ | contains 1 additional Fe2+ besides the [4Fe-4S]-cluster | Pyrococcus furiosus | |
Mg2+ | both enzyme forms | Pyrococcus furiosus | |
Mg2+ | contains 1 Mg-atom per subunit | Pyrococcus furiosus | |
Mg2+ | contains 1 Mg-atom per subunit | Thermococcus sp. | |
Tungsten | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus furiosus | |
Tungsten | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus sp. | |
Tungsten | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus litoralis | |
Tungsten | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus endeavori | |
Tungsten | 1 tungsten per subunit | Pyrococcus furiosus | |
Tungsten | 1 tungsten per subunit | Thermococcus sp. | |
Tungsten | 1 tungsten per subunit | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Thermococcus sp. |
67000 | - |
2 * 67000 | Pyrococcus furiosus |
136100 | - |
- |
Pyrococcus furiosus |
280000 | - |
- |
Pyrococcus furiosus |
280000 | - |
- |
Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | Thermococcus sp. | - |
an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | Pyrococcus endeavori | - |
an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | Pyrococcus furiosus | formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | Thermococcus litoralis | formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | Thermococcus sp. ES1 | - |
an acid + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus endeavori | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Pyrococcus furiosus | - |
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase | - |
Pyrococcus furiosus | Q51739 | hyperthermophilic archaeon | - |
Pyrococcus furiosus | Q51739 | strain DSM 3638 | - |
Pyrococcus furiosus | Q51739 | gene aor | - |
Thermococcus litoralis | - |
hyperthermophilic archaeon | - |
Thermococcus litoralis | - |
formaldehyde ferredoxin oxidoreductase | - |
Thermococcus sp. | - |
hyperthermophilic archaeon | - |
Thermococcus sp. ES1 | - |
hyperthermophilic archaeon | - |
Oxidation Stability | Organism |
---|---|
aldehyde ferredoxin oxidoreductase, t1/2: 30 min at 23 °C | Pyrococcus furiosus |
formaldehyde ferredoxin oxidoreductase, t1/2: 9 h at 23°C | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
formaldehyde and aldehyde ferredoxin oxidoreductase | Pyrococcus furiosus |
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required | Pyrococcus furiosus |
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | acetate + H+ + reduced benzyl viologen | - |
ir | |
acetaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | acetate + H+ + reduced benzyl viologen | - |
ir | |
acetaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | acetate + H+ + reduced benzyl viologen | - |
ir | |
acetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. ES1 | acetate + H+ + reduced benzyl viologen | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. | an acid + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus litoralis | an acid + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus endeavori | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus endeavori | an acid + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea | Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea | Thermococcus litoralis | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. ES1 | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. ES1 | an acid + H+ + reduced ferredoxin | - |
ir | |
benzaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | benzoic acid + H+ + reduced benzyl viologen | - |
ir | |
benzaldehyde + H2O + oxidized benzyl viologen | no activity | Thermococcus litoralis | benzoic acid + H+ + reduced benzyl viologen | - |
? | |
crotonaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | crotonate + H+ + reduced benzyl viologen | - |
ir | |
crotonaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | crotonate + H+ + reduced benzyl viologen | - |
ir | |
crotonaldehyde + H2O + oxidized benzyl viologen | no activity | Thermococcus litoralis | crotonate + H+ + reduced benzyl viologen | - |
? | |
crotonaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. ES1 | crotonate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | formate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | formate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | formate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. ES1 | formate + H+ + reduced benzyl viologen | - |
ir | |
glutaraldehyde + H2O + oxidized methyl viologen | - |
Pyrococcus furiosus | glutarate + H+ + reduced methyl viologen | - |
? | |
glutaric dialdehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | ? | - |
ir | |
indoleacetaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | indoleacetate + H+ + reduced benzyl viologen | - |
? | |
indoleacetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | indoleacetate + H+ + reduced benzyl viologen | - |
ir | |
indoleacetaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | indoleacetate + H+ + reduced benzyl viologen | - |
ir | |
isovalerylaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | isovalerate + H+ + reduced benzyl viologen | - |
ir | |
isovalerylaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase, no activity | Pyrococcus furiosus | isovalerate + H+ + reduced benzyl viologen | - |
? | |
isovalerylaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase, no activity | Thermococcus litoralis | isovalerate + H+ + reduced benzyl viologen | - |
? | |
phenylacetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | phenylacetate + H+ + reduced benzyl viologen | - |
ir | |
phenylacetaldehyde + H2O + oxidized benzyl viologen | no activity | Thermococcus litoralis | phenylacetate + H+ + reduced benzyl viologen | - |
? | |
phenylpropionaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | phenylpropanoate + H+ + reduced benzyl viologen | - |
r | |
phenylpropionaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | phenylpropanoate + H+ + reduced benzyl viologen | - |
ir | |
propionaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | propionate + H+ + reduced benzyl viologen | - |
ir | |
propionaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | propionate + H+ + reduced benzyl viologen | - |
ir | |
propionaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | propionate + H+ + reduced benzyl viologen | - |
ir | |
succinic semialdehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | succinic acid + H+ + reduced benzyl viologen | - |
r | |
succinic semialdehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | succinic acid + H+ + reduced benzyl viologen | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pyrococcus furiosus |
dimer | 2 * 67000, SDS-PAGE | Thermococcus sp. |
dimer | 2 * 67000 | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
More | formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
80 | - |
assay at | Thermococcus sp. |
80 | - |
assay at | Thermococcus litoralis |
80 | - |
assay at | Pyrococcus endeavori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
formaldehyde ferredoxin oxidoreductase, t1/2: 6 h | Pyrococcus furiosus |
80 | - |
aldehyde ferredoxin oxidoreductase, t1/2: 15 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pyrococcus furiosus |
8.4 | - |
assay at | Thermococcus sp. |
8.4 | - |
assay at | Thermococcus litoralis |
8.4 | - |
assay at | Pyrococcus endeavori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
Ferredoxin | - |
Thermococcus sp. | |
Ferredoxin | - |
Thermococcus litoralis | |
Ferredoxin | - |
Pyrococcus endeavori | |
tungsten cofactor | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
tungsten cofactor | formaldehyde ferredoxin oxidoreductase | Thermococcus litoralis | |
tungsten cofactor | pterin ring system | Pyrococcus furiosus | |
tungsten cofactor | pterin ring system | Thermococcus sp. | |
tungsten cofactor | pterin ring system | Thermococcus litoralis | |
tungsten cofactor | pterin ring system | Pyrococcus endeavori | |
tungsten-molybdopterin | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus furiosus | |
tungsten-molybdopterin | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus sp. | |
tungsten-molybdopterin | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Thermococcus litoralis | |
tungsten-molybdopterin | in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues | Pyrococcus endeavori |