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Literature summary for 1.2.7.5 extracted from

  • Hu, Y.; Faham, S.; Roy, R.; Adams, M.W.; Rees, D.C.
    Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus: the 1.85 A resolution crystal structure and its mechanistic implications (1999), J. Mol. Biol., 286, 899-914.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
formaldehyde ferredoxin oxidoreductase, purified enzyme, modified melting-point capillary method, room temperature under argon atmosphere, protein solution: 55-65 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM dithionite, 2 mM DTT, 0.2 M KCl, precipitant solution: 30% v/v glycerol, 20% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, 0.2 M NaCl, several weeks, X-ray structure determination and analysis Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ binding site, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
Fe2+ the [4Fe-4S]-cluster is coordinated by Cys287 of the formaldehyde ferredoxin oxidoreductase and Asp14 of ferredoxin, structure Pyrococcus furiosus
Tungsten
-
Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
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hyperthermophilic archaeon
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Pyrococcus furiosus
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Protein Data Bank entries 1B25 and 1B4N
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Pyrococcus furiosus
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contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
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Reaction

Reaction Comment Organism Reaction ID
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin active site structure Pyrococcus furiosus
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin intermolecular interaction analysis Pyrococcus furiosus
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin substrate binding, catalytic site and reaction mechanism including cofactor action Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes Pyrococcus furiosus an acid + H+ + reduced ferredoxin
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ir

Synonyms

Synonyms Comment Organism
More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin formaldehyde ferredoxin oxidoreductase, binding mechanism Pyrococcus furiosus
tungsten cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
tungsten-molybdopterin tungstopterin center Pyrococcus furiosus