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Literature summary for 1.2.7.5 extracted from

  • Chan, M.K.; Mukund, S.; Kletzin, A.; Adams, M.W.; Rees, D.C.
    Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase (1995), Science, 267, 1463-1469.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure determination and analysis Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
Molybdenum part of a tungsten-molybdopterin cofactor Pyrococcus furiosus
Tungsten part of a tungsten-molybdopterin cofactor Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, crystal structure Pyrococcus furiosus
134000
-
crystal structure Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
an acid + H+ + reduced ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
hyperthermophilic archaeon
-

Reaction

Reaction Comment Organism Reaction ID
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin substrate binding, catalytic site and reaction mechanism including cofactor action Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin
-
Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
?
an aldehyde + H2O + oxidized ferredoxin
-
Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 66000, crystal structure Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
above Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme is thermostable Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Pyrococcus furiosus
tungsten-molybdopterin tungstopterin center Pyrococcus furiosus
tungsten-molybdopterin structural coordination of the tungsten by 4 sulfur ligands in the cofactor Pyrococcus furiosus
tungsten-molybdopterin interaction with of DXXGL(D,C)X motif and Gly341, Asp343, Asp338, Gly492, Cys494, and Asp489 Pyrococcus furiosus