Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure determination and analysis | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Pyrococcus furiosus | |
Molybdenum | part of a tungsten-molybdopterin cofactor | Pyrococcus furiosus | |
Tungsten | part of a tungsten-molybdopterin cofactor | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, crystal structure | Pyrococcus furiosus |
134000 | - |
crystal structure | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | Pyrococcus furiosus | - |
an acid + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | substrate binding, catalytic site and reaction mechanism including cofactor action | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, crystal structure | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
above | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermostable | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus | |
tungsten-molybdopterin | structural coordination of the tungsten by 4 sulfur ligands in the cofactor | Pyrococcus furiosus | |
tungsten-molybdopterin | interaction with of DXXGL(D,C)X motif and Gly341, Asp343, Asp338, Gly492, Cys494, and Asp489 | Pyrococcus furiosus |