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Literature summary for 1.2.7.5 extracted from

  • Mukund, S.; Adams, M.W.W.
    The novel tungsten-iron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase. Evidence for its participation in a unique glycolytic pathway (1991), J. Biol. Chem., 266, 14208-14216.
    View publication on PubMed
Search for more literature for 1.2.7.5

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect of CoA Pyrococcus furiosus

General Stability

General Stability Organism
10% glycerol and 2 mM DTT stabilize during purification Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
arsenite 50% inhibition at 1 mM Pyrococcus furiosus
crotonaldehyde substrate inhibition above 0.2 mM Pyrococcus furiosus
cyanide 50% inhibition at 8 mM Pyrococcus furiosus
iodoacetate 50% inhibition at 0.2 mM Pyrococcus furiosus
additional information no effect of CoA Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 crotonaldehyde pH 8.4, 80°C, with methyl viologen as electron acceptor Pyrococcus furiosus
1
-
 glyceraldehyde pH 8.4, 65°C, with methyl viologen as electron acceptor Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 6.6 gatoms per 85000g of protein Pyrococcus furiosus
Molybdenum part of a tungsten-molybdopterin cofactor Pyrococcus furiosus
Tungsten part of a tungsten-molybdopterin cofactor Pyrococcus furiosus
Tungsten 1.0 gatoms per 85000g of protein Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 1 * 80000, SDS-PAGE Pyrococcus furiosus
90000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 an acid + H+ + reduced ferredoxin
-
 ?
glyceraldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus involved in glycolysis glycerate + H+ + reduced ferredoxin
-
 ir

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

Oxidation Stability Organism
sensitive against O2, dithionite is added to avoid trace contamination with O2 during purification and enzyme assay Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity under strict anaerobic conditions Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53.6
-
 purified enzyme, substrate crotonaldehyde with methyl viologen as electron acceptor Pyrococcus furiosus

Storage Stability

Storage Stability Organism
-196°C, pure enzyme pellet is thawed anaerobically after storage in liquid N2, no loss of activity for several months Pyrococcus furiosus
4°c or 23°C, pure enzyme, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, loss of 25% activity after 6 h under strict anaerobic conditions Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus acetate + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ir
butyraldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus butyrate + H+ + reduced ferredoxin
-
 ir
crotonaldehyde + H2O + oxidized ferredoxin best substrate Pyrococcus furiosus crotonate + H+ + reduced ferredoxin
-
 ir
crotonaldehyde + H2O + oxidized methyl viologen artifical electron acceptor Pyrococcus furiosus crotonate + H+ + reduced methyl viologen
-
 ir
formaldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus formate + H+ + reduced ferredoxin
-
 ir
glyceraldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus glycerate + H+ + reduced ferredoxin
-
 ir
glyceraldehyde + H2O + oxidized ferredoxin involved in glycolysis Pyrococcus furiosus glycerate + H+ + reduced ferredoxin
-
 ir
glyceraldehyde + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus glycerate + H+ + reduced methyl viologen
-
 ir
additional information enzyme shows an active and an inactive form Pyrococcus furiosus ?
-
 ?
additional information no oxidizing activity with glyceraldehyde-3-phosphate, glyoxylate, glucose, glucose 6-phosphate, CO, H2, formate, pyruvate, 2-oxoglutarate Pyrococcus furiosus ?
-
 ?
additional information NAD(P) is no electron acceptor Pyrococcus furiosus ?
-
 ?
additional information no H2 evolution from reduced methyl viologen Pyrococcus furiosus ?
-
 ?
additional information no activity with CoA Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 above Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme is thermostable Pyrococcus furiosus
23
-
 pure enzyme, 5.2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, less than 20% remaining activity after 5 min, after 10 additional min 10% residual activity is reached, which stays constant for more than 30 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 sharp maximum Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.4 activity increases with increasing pH at increasing temperature, nearly no activity below pH 7.0 Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Pyrococcus furiosus
tungsten-molybdopterin tungstopterin center Pyrococcus furiosus