BRENDA - Enzyme Database show
show all sequences of 1.2.7.5

Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1

Heider, J.; Ma, K.; Adams, M.W.W.; J. Bacteriol. 177, 4757-4764 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridyl
competitive to methyl viologen
Thermococcus sp.
acetaldehyde
50% inhibition at 5 mM
Thermococcus sp.
aldehydes
substrate inhibition at high concentrations, overview
Thermococcus sp.
arsenite
50% inhibition at 0.5 mM
Thermococcus sp.
crotonaldehyde
50% inhibition at 20 mM
Thermococcus sp.
iodoacetate
50% inhibition at 0.5 mM
Thermococcus sp.
isovalerylaldehyde
50% inhibition at 4 mM
Thermococcus sp.
p-chloromercuribenzoate
-
Thermococcus sp.
phenylacetaldehyde
50% inhibition at 7 mM
Thermococcus sp.
propionaldehyde
50% inhibition at 0.6 mM
Thermococcus sp.
Zn2+
50% inhibition at 0.25 mM
Thermococcus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
Ferredoxin
ES-1 ferredoxin; pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
0.016
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.028
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.05
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.057
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.065
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.076
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.13
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
0.14
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.15
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.17
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
0.2
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.42
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.8
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
2.9
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
0.7 gatoms per subunit
Thermococcus sp.
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit
Thermococcus sp.
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
Molybdenum
-
Thermococcus sp.
Tungsten
1 tungsten per subunit
Thermococcus sp.
Zn2+
0.2 gatoms per subunit
Thermococcus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
105000
-
gel filtration
Thermococcus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
enzyme oxidizes aldehydes generated during amino acid catabolism
an acid + H+ + reduced ferredoxin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus sp.
-
hyperthermophilic archaeon
-
Thermococcus sp. ES1
-
hyperthermophilic archaeon
-
Oxidation Stability
Oxidation Stability
Organism
very sensitive to O2, after 1 h at 60C 3% activity remains, after 1 h at 4C 39% activity remains
Thermococcus sp.
Purification (Commentary)
Commentary
Organism
about 120fold, to homogeneity
Thermococcus sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
238
-
purified enzyme
Thermococcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
acetaldehyde + H2O + oxidized ferredoxin
best substrate
11935
Thermococcus sp.
acetate + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
acetate + H+ + reduced methyl viologen
very low activity
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
acetate + H+ + reduced methyl viologen
below pH 6.0
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
-
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
enzyme oxidizes aldehydes generated during amino acid catabolism
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
-
-
?
benzaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
formaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
glyceraldehyde + H2O + oxidized methyl viologen
very poor substrate
11935
Thermococcus sp.
glycerate + H+ + reduced methyl viologen
-
-
-
-
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
additional information
acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate
11935
Thermococcus sp.
?
-
-
-
-
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
propionaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
salicylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
salicylic acid + H+ + reduced benzyl viologen
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
assay at
Thermococcus sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the enzyme is thermostable
Thermococcus sp.
23
-
pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min
Thermococcus sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
2.8
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
13
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
22
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
55
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
62
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
250
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
269
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
270
-
Ferredoxin
ES-1 ferredoxin, pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
272
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
343
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
720
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
950
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
960
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1100
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Thermococcus sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
oxidation of acetaldehyde with benzyl viologen as electron acceptor
Thermococcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Thermococcus sp.
form A cofactor
in presence of iodine
Thermococcus sp.
form B cofactor
in absence of iodine
Thermococcus sp.
molybdopterin
-
Thermococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Thermococcus sp.
form A cofactor
in presence of iodine
Thermococcus sp.
form B cofactor
in absence of iodine
Thermococcus sp.
molybdopterin
-
Thermococcus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridyl
competitive to methyl viologen
Thermococcus sp.
acetaldehyde
50% inhibition at 5 mM
Thermococcus sp.
aldehydes
substrate inhibition at high concentrations, overview
Thermococcus sp.
arsenite
50% inhibition at 0.5 mM
Thermococcus sp.
crotonaldehyde
50% inhibition at 20 mM
Thermococcus sp.
iodoacetate
50% inhibition at 0.5 mM
Thermococcus sp.
isovalerylaldehyde
50% inhibition at 4 mM
Thermococcus sp.
p-chloromercuribenzoate
-
Thermococcus sp.
phenylacetaldehyde
50% inhibition at 7 mM
Thermococcus sp.
propionaldehyde
50% inhibition at 0.6 mM
Thermococcus sp.
Zn2+
50% inhibition at 0.25 mM
Thermococcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
Ferredoxin
ES-1 ferredoxin; pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
0.016
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.028
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.05
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.057
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.065
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.076
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.13
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
0.14
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.15
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
0.17
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
0.2
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
1.42
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1.8
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
2.9
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
0.7 gatoms per subunit
Thermococcus sp.
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit
Thermococcus sp.
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
Molybdenum
-
Thermococcus sp.
Tungsten
1 tungsten per subunit
Thermococcus sp.
Zn2+
0.2 gatoms per subunit
Thermococcus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
105000
-
gel filtration
Thermococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
enzyme oxidizes aldehydes generated during amino acid catabolism
an acid + H+ + reduced ferredoxin
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
very sensitive to O2, after 1 h at 60C 3% activity remains, after 1 h at 4C 39% activity remains
Thermococcus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
about 120fold, to homogeneity
Thermococcus sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
238
-
purified enzyme
Thermococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
acetaldehyde + H2O + oxidized ferredoxin
best substrate
11935
Thermococcus sp.
acetate + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
acetate + H+ + reduced methyl viologen
very low activity
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
acetate + H+ + reduced methyl viologen
below pH 6.0
11935
Thermococcus sp.
acetaldehyde + H2O + oxidized methyl viologen
-
11935
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
-
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
11935
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
enzyme oxidizes aldehydes generated during amino acid catabolism
11935
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
-
-
?
benzaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
formaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
glyceraldehyde + H2O + oxidized methyl viologen
very poor substrate
11935
Thermococcus sp.
glycerate + H+ + reduced methyl viologen
-
-
-
-
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
additional information
acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate
11935
Thermococcus sp.
?
-
-
-
-
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
propionaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
11935
Thermococcus sp.
ir
salicylaldehyde + H2O + oxidized benzyl viologen
-
11935
Thermococcus sp.
salicylic acid + H+ + reduced benzyl viologen
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
assay at
Thermococcus sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the enzyme is thermostable
Thermococcus sp.
23
-
pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min
Thermococcus sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6
-
acetate
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
2.8
-
glyceraldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
13
-
Salicylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
22
-
crotonaldehyde
pH 8.4, 45C, benzyl viologen as electron acceptor
Thermococcus sp.
55
-
Indoleacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
62
-
methyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
250
-
benzyl viologen
pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
269
-
crotonaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
270
-
Ferredoxin
ES-1 ferredoxin, pH 8.4, 85C, with crotonaldehyde
Thermococcus sp.
272
-
isovalerylaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
343
-
acetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
720
-
benzaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
950
-
formaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
960
-
phenylacetaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
1100
-
propionaldehyde
pH 8.4, 85C, benzyl viologen as electron acceptor
Thermococcus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Thermococcus sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
oxidation of acetaldehyde with benzyl viologen as electron acceptor
Thermococcus sp.
Other publictions for EC 1.2.7.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740953
Liew
Metabolic engineering of Clost ...
Clostridium autoethanogenum
Metab. Eng.
40
104-114
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
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-
-
-
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1
-
-
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-
-
-
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-
-
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1
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1
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-
-
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1
1
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-
-
740209
Ammam
Effect of tungstate on acetate ...
Sporomusa ovata, Sporomusa ovata DSM-2662
Biotechnol. Biofuels
9
163
2016
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-
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1
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2
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4
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2
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1
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2
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2
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737164
Basen
Single gene insertion drives b ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
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Simultaneous involvement of a ...
Aromatoleum aromaticum
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Why is the molybdenum-substitu ...
Pyrococcus furiosus
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Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
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745210
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Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
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692610
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In situ generation of oxo-sulf ...
synthetic construct
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Thapper
Biochemical and spectroscopic ...
Desulfovibrio aminophilus, Desulfovibrio aminophilus DSM 12254
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44-50
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656293
Hagedoorn
Purification and characterizat ...
Pyrobaculum aerophilum
J. Biol. Inorg. Chem.
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259-269
2005
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674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
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2005
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654430
Wahyudi
Characterization of aldehyde f ...
Magnetospirillum magneticum, Magnetospirillum magneticum AMB-1
Biochem. Biophys. Res. Commun.
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223-229
2003
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus endeavori, Pyrococcus furiosus, Thermococcus litoralis, Thermococcus sp., Thermococcus sp. ES1
Methods Enzymol.
331
132-144
2001
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
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1171-1180
1999
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644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
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899-914
1999
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721578
Zhou
Site-directed mutations of the ...
Pyrococcus furiosus
Biochemistry
36
10892-10900
1997
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644687
Koehler
-
Spectroscopic characterization ...
Pyrococcus endeavori, Pyrococcus furiosus
J. Am. Chem. Soc.
118
12391-12405
1996
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644688
Das
-
Modeling the tungsten sites of ...
Pyrococcus furiosus
J. Am. Chem. Soc.
118
1387-1397
1996
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722057
Arendsen
-
Redox chemistry of biological ...
Pyrococcus furiosus
Chemistry
1
292-296
1996
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722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
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11935
Heider
Purification, characterization ...
Thermococcus sp., Thermococcus sp. ES1
J. Bacteriol.
177
4757-4764
1995
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644684
Kletzin
Molecular characterization of ...
Pyrococcus furiosus
J. Bacteriol.
177
4817-4819
1995
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644686
Chan
Structure of a hyperthermophil ...
Pyrococcus furiosus
Science
267
1463-1469
1995
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722438
George
-
Aldehyde ferredoxin oxidoreduc ...
Pyrococcus furiosus
J. Am. Chem. Soc.
114
3521-3523
1992
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11938
Mukund
The novel tungsten-iron-sulfur ...
Pyrococcus furiosus
J. Biol. Chem.
266
14208-14216
1991
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