KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0107 | - |
methylene blue | at pH 7.2 and 25°C | Afipia carboxidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | contains copper, essential for activity | Afipia carboxidovorans | |
Molybdenum | contains molybdenum, essential for activity | Afipia carboxidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + acceptor | Afipia carboxidovorans | - |
CO2 + reduced acceptor + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Afipia carboxidovorans | P19919 and P19920 and P19921 | P19919 large subunit, P19920 medium subunit, P19921 small subunit | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration | Afipia carboxidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + acceptor | - |
Afipia carboxidovorans | CO2 + reduced acceptor + H+ | - |
? | |
CO + H2O + methylene blue | - |
Afipia carboxidovorans | CO2 + reduced methylene blue | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CO dehydrogenase | - |
Afipia carboxidovorans |
CODH | - |
Afipia carboxidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
57.8 | - |
methylene blue | at pH 7.2 and 25°C | Afipia carboxidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Afipia carboxidovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Afipia carboxidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Afipia carboxidovorans | |
Fe-S center | - |
Afipia carboxidovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8700 | - |
methylene blue | at pH 7.2 and 25°C | Afipia carboxidovorans |