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Literature summary for 1.2.7.4 extracted from
- New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster (2005), J. Biol. Inorg. Chem., 10, 903-912.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C338A | complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
| C451A | complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
| C451S | 1.4% of wild-type specific activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
| C481A | unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
| C531A | 0.1% of wild-type specific activity | Rhodospirillum rubrum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
CO | mutant C451S, pH 7.5 | Rhodospirillum rubrum |  |
| 0.032 | - |
CO | wild-type, pH 7.5 | Rhodospirillum rubrum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rhodospirillum rubrum | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | wild-type, 8.97 mol per mol of enzyme | Rhodospirillum rubrum | |
| Nickel | wild-type, 0.85 mol per mol of enzyme. Analysis of activation of wild-type and mutant apo-enzymes by nickel | Rhodospirillum rubrum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
mutant C531A, pH 7.5 | Rhodospirillum rubrum |
| 86.3 | - |
mutant C451S, pH 7.5 | Rhodospirillum rubrum |
| 6230 | - |
wild-type, pH 7.5 | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO + H2O + methyl viologen | - |
Rhodospirillum rubrum | CO2 + reduced methyl viologen | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
mutant C451S | Rhodospirillum rubrum |
| 8.6 | - |
wild-type | Rhodospirillum rubrum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | wild-type and mutants apo-enzymes, stable for at least 30 min within this range | Rhodospirillum rubrum |
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Release 2023.1 (January 2023)
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