Literature summary for 1.2.7.4 extracted from

Lorite, M.J.; Tachil, J.; Sanjuan, J.; Meyer, O.; Bedmar, E.J.
Carbon monoxide dehydrogenase activity in Bradyrhizobium japonicum (2000), Appl. Environ. Microbiol., 66, 1871-1876.

Search for more literature for 1.2.7.4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Molybdenum	2.29 mol/mol enzyme	Bradyrhizobium japonicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17200	-	L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE	Bradyrhizobium japonicum
28400	-	L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE	Bradyrhizobium japonicum
75000	-	L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE	Bradyrhizobium japonicum
230000	-	native PAGE	Bradyrhizobium japonicum
235000	-	gel filtration	Bradyrhizobium japonicum

Organism

Organism	UniProt	Comment	Textmining
Bradyrhizobium japonicum	-	gram-negativ soil bacterium	-

Purification (Commentary)

Purification (Comment)	Organism
anion-exchange, hydrophobic interaction, gel filtration	Bradyrhizobium japonicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0577	-	-	Bradyrhizobium japonicum

Subunits

Subunits	Comment	Organism
hexamer	L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE	Bradyrhizobium japonicum

Cofactor

Cofactor	Comment	Organism	Structure
flavin adenine dinucleotide	1.99 mol flavin/mol enzyme	Bradyrhizobium japonicum
molybdopterin cytosine dinucleotide	-	Bradyrhizobium japonicum

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Release 2023.1 (January 2023)