General Stability | Organism |
---|---|
72 h exposure to air at 4°C, about 10% loss of activity | Methanothrix soehngenii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | 0.2 mM, 17% inhibition in the presence of air, 32% CO and 69% N2, inhibition can be removed by O2 or CO | Methanothrix soehngenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
methyl viologen | - |
Methanothrix soehngenii | |
0.7 | - |
CO | - |
Methanothrix soehngenii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19400 | - |
2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
79400 | - |
2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
190000 | - |
gel filtration | Methanothrix soehngenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothrix soehngenii | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen stable, 10% loss in activity after 72 h exposure to air at 4°C | Methanothrix soehngenii |
Purification (Comment) | Organism |
---|---|
- |
Methanothrix soehngenii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117 | - |
- |
Methanothrix soehngenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + coenzyme F420 | 1.5% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + reduced coenzyme F420 | - |
? | |
CO + H2O + FAD | 0.3% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + FADH2 | - |
? | |
CO + H2O + FMN | 0.6% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + FMNH2 | - |
? | |
CO + H2O + methyl viologen | - |
Methanothrix soehngenii | CO2 + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, about 10% loss of activity | Methanothrix soehngenii |
72 | - |
10 min, complete inactivation | Methanothrix soehngenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Methanothrix soehngenii |