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Literature summary for 1.2.7.4 extracted from

  • Jetten, M.S.M.; Stams, A.J.M.; Zehnder, A.J.B.
    Purification and characterization of an oxygen-stable carbon monoxide dehydrogenase of Methanothrix soehngenii (1989), Eur. J. Biochem., 181, 437-441.
    View publication on PubMed

General Stability

General Stability Organism
72 h exposure to air at 4°C, about 10% loss of activity Methanothrix soehngenii

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.2 mM, 17% inhibition in the presence of air, 32% CO and 69% N2, inhibition can be removed by O2 or CO Methanothrix soehngenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
methyl viologen
-
Methanothrix soehngenii
0.7
-
CO
-
Methanothrix soehngenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19400
-
2 * 19400 + 2 * 79400, SDS-PAGE Methanothrix soehngenii
79400
-
2 * 19400 + 2 * 79400, SDS-PAGE Methanothrix soehngenii
190000
-
gel filtration Methanothrix soehngenii

Organism

Organism UniProt Comment Textmining
Methanothrix soehngenii
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen stable, 10% loss in activity after 72 h exposure to air at 4°C Methanothrix soehngenii

Purification (Commentary)

Purification (Comment) Organism
-
Methanothrix soehngenii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
117
-
-
Methanothrix soehngenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + coenzyme F420 1.5% of the activity with methyl viologen Methanothrix soehngenii CO2 + reduced coenzyme F420
-
?
CO + H2O + FAD 0.3% of the activity with methyl viologen Methanothrix soehngenii CO2 + FADH2
-
?
CO + H2O + FMN 0.6% of the activity with methyl viologen Methanothrix soehngenii CO2 + FMNH2
-
?
CO + H2O + methyl viologen
-
Methanothrix soehngenii CO2 + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 19400 + 2 * 79400, SDS-PAGE Methanothrix soehngenii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, about 10% loss of activity Methanothrix soehngenii
72
-
10 min, complete inactivation Methanothrix soehngenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Methanothrix soehngenii