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Literature summary for 1.2.7.4 extracted from
- Carbon monoxide dehydrogenase from Methanosarcina barkeri. Disaggregation, purification, and physicochemical properties of the enzyme (1987), J. Biol. Chem., 262, 3706-3712.
General Stability
| General Stability | Organism |
|---|---|
| CO renders the enzyme more susceptible to temperature inactivation | Methanosarcina barkeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Butanedione | - |
Methanosarcina barkeri |  |
| CN- | - |
Methanosarcina barkeri | |
| Glyoxaldehyde | inactivation requires enzymatic turnover; only with CO and methyl viologen as substrates | Methanosarcina barkeri | |
| KCN | - |
Methanosarcina barkeri | |
| additional information | dimethylglyoxime is no inhibitor, formaldehyde and acetaldehyde does not inactivate the enzyme; not inhibited by 20 mM formaldehyde or acetaldehyde | Methanosarcina barkeri | |
| O2 | - |
Methanosarcina barkeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
2,3,5-triphenyltetrazolium chloride | below 0.5 mM | Methanosarcina barkeri | |
| 0.5 | - |
2,3,5-triphenyltetrazolium chloride | pH 7.0, 25°C | Methanosarcina barkeri | |
| 7.1 | - |
methyl viologen | - |
Methanosarcina barkeri | |
| 7.1 | - |
methyl viologen | pH 7.0, 25°C | Methanosarcina barkeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | tightly bound by the enzyme | Methanosarcina barkeri | |
| Iron | contains 15.6 mol of iron per mol of alpha2beta oligomer | Methanosarcina barkeri | |
| additional information | no stimulation by MgSO4, CoCl2, NiCl2 and ZnSO4 | Methanosarcina barkeri | |
| Ni2+ | tightly bound by the enzyme | Methanosarcina barkeri | |
| Nickel | contains 1.3 mol of nickel per mol of alpha2beta2 oligomer | Methanosarcina barkeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19700 | - |
2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
| 19700 | - |
2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
| 84500 | - |
2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
| 84500 | - |
2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
| 161000 | - |
gel filtration | Methanosarcina barkeri |
| 161000 | - |
dialyzed enzyme, gel filtration | Methanosarcina barkeri |
| 205000 | - |
gradient gel electrophoresis | Methanosarcina barkeri |
| 3000000 | - |
high molecular weight form exists under conditions of high ionic strength, gel filtration | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO + H2O + oxidized ferredoxin | Methanosarcina barkeri | may participate in methanogenesis by cleavage of acetate, reverse of the reaction in acetate biosynthesis | CO2 + reduced ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| extremely sensitive to air, most of the enzyme activity is lost upon exposure to air for 1 min, longer incubation results in complete inactivation | Methanosarcina barkeri |
| strong inhibition by oxygen, extremely sensitive, exposed to air activity is lost in less than 1 min | Methanosarcina barkeri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Methanosarcina barkeri | - |
| culture condition:ammonium malate-grown cell | - |
Methanosarcina barkeri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 133.6 | - |
- |
Methanosarcina barkeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, samples thawed under anaerobic conditions exhibits full activity even after 6 months of storage | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO + H2O + 2,3,5-triphenyltetrazolium chloride | - |
Methanosarcina barkeri | CO2 + reduced 2,3,5-triphenyltetrazolium chloride | - |
? | |
| CO + H2O + FAD | - |
Methanosarcina barkeri | CO2 + FADH2 | - |
? | |
| CO + H2O + ferredoxin | - |
Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
| CO + H2O + FMN | - |
Methanosarcina barkeri | CO2 + FMNH2 | - |
? | |
| CO + H2O + methyl viologen | the enzyme fails to reduce NAD+, NADP+, or 8-hydroxy-5-deazaflavin cofactor 420 | Methanosarcina barkeri | CO2 + reduced methyl viologen | - |
? | |
| CO + H2O + oxidized 2,3,5-triphenyltetrazolium chloride | - |
Methanosarcina barkeri | CO2 + reduced 2,3,5-triphenyltetrazolium chloride | - |
? | |
| CO + H2O + oxidized ferredoxin | - |
Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
| CO + H2O + oxidized ferredoxin | may participate in methanogenesis by cleavage of acetate, reverse of the reaction in acetate biosynthesis | Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
| CO + H2O + oxidized flavodoxin | - |
Methanosarcina barkeri | CO2 + reduced flavodoxin | - |
? | |
| CO + H2O + oxidized methyl viologen | - |
Methanosarcina barkeri | CO2 + reduced methyl viologen | - |
? | |
| CO + H2O + oxidized phenazine methosulfate | - |
Methanosarcina barkeri | CO2 + reduced phenazine methosulfate | - |
? | |
| CO + H2O + phenazine methosulfate | - |
Methanosarcina barkeri | CO2 + reduced phenazine methosulfate | - |
? | |
| additional information | enzyme fails to reduce NAD+, NADP+ or the 8-hydroxy-5-deazaflavin factor F420 | Methanosarcina barkeri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
| tetramer | 2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
CO renders the enzyme more susceptible to temperature inactivation | Methanosarcina barkeri |
| 24 | - |
10 min, about 30% loss of activity in presence of CO, stable in absence of CO | Methanosarcina barkeri |
| 39 | - |
10 min, 45% loss of activity in presence of CO, stable in absence of CO | Methanosarcina barkeri |
| 62 | - |
10 min, about 65% loss of activity in presence of CO, about 5% loss of activity in absence of CO | Methanosarcina barkeri |
| 80 | - |
10 min, about 85% loss of activity in presence of CO, about 20% loss of activity in absence of CO | Methanosarcina barkeri |
| 80 | 104 | most of the activity remains after heating at 80°C, nearly complete inactivation after heating at 104°C, greater loss of activity at all temperatures when heated in presence of CO | Methanosarcina barkeri |
| 104 | - |
10 min, complete loss of activity in presence or in absence of CO | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Methanosarcina barkeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | very little activity outside this range | Methanosarcina barkeri |
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