General Stability | Organism |
---|---|
Triton X-100 highly stablizes during purification | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
CoA | pH 7.6, 70°C | Hydrogenobacter thermophilus | |
1.42 | - |
2-oxoglutarate | pH 7.6, 70°C | Hydrogenobacter thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Hydrogenobacter thermophilus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE | Hydrogenobacter thermophilus |
70000 | - |
1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE | Hydrogenobacter thermophilus |
105000 | - |
gel filtration | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + CO2 + reduced ferredoxin | Hydrogenobacter thermophilus | involved in reductive TCA-cycle | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r | |
succinyl-CoA + CO2 + reduced ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | involved in reductive TCA-cycle | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
pH 7.6, 70°C | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + oxidized ferredoxin | no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
r | |
2-oxoglutarate + CoA + oxidized ferredoxin | no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
r | |
succinyl-CoA + CO2 + reduced ferredoxin | - |
Hydrogenobacter thermophilus | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r | |
succinyl-CoA + CO2 + reduced ferredoxin | involved in reductive TCA-cycle | Hydrogenobacter thermophilus | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r | |
succinyl-CoA + CO2 + reduced ferredoxin | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r | |
succinyl-CoA + CO2 + reduced ferredoxin | involved in reductive TCA-cycle | Hydrogenobacter thermophilus TK-6 / IAM 12695 | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
2-oxoglutarate:ferredoxin oxidoreductase | - |
Hydrogenobacter thermophilus |
OGOR | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 85 | - |
Hydrogenobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
22 h, anaerobic conditions, 50% loss of activity | Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 7.8 | 2-oxoglutarate oxidation reaction | Hydrogenobacter thermophilus |