KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
0.8 | - |
methylviologen | - |
Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 8 mol of non-heme iron and 8 mol of acid-labile sulfur per mol of enzyme | Methanothermobacter thermautotrophicus | |
Tungsten | tungsten-containing isoenzyme: contains 0.4 mol of tungsten per mol of enzyme | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
31000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
53000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
65000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
160000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
tungsten-containing isoenzyme | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus | methanofuran + reduced methylviologen + CO2 | - |
? | |
formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methanofuran + reduced methylviologen + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pterin | contains molybdopterin guanine dinucleotide as pterin cofactor | Methanothermobacter thermautotrophicus |