General Stability | Organism |
---|---|
enzyme in crude enzyme extract is unstable to freezing | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N-formylmethanofuran | molybdenum enzyme | Methanosarcina barkeri | |
0.02 | - |
N-formylmethanofuran | value below, methylviologen, molybdenum enzyme | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 28 mol of non-heme iron and acid labile sulfur per mol of 220000 MW enzyme | Methanosarcina barkeri | |
Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanosarcina barkeri | |
Molybdenum | contains 0.5-0.8 mol of molybdenum per mol of 220000 MW enzyme | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
29000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
34000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
37000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
50000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
65000 | - |
x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
200000 | - |
and MW 400000, native PAGE | Methanosarcina barkeri |
400000 | - |
and MW 200000, native PAGE | Methanosarcina barkeri |
440000 | - |
gel filtration | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formylmethanofuran + oxidized methylviologen + H2O | - |
Methanosarcina barkeri | methanofuran + reduced methylviologen + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE | Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
640 | - |
formylmethanofuran | - |
Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pterin | contains 0.6-0.9 mol of pterin per mol of 22000 MW enzyme | Methanosarcina barkeri |