Any feedback?
Literature summary for 1.2.7.12 extracted from
- A molybdenum and a tungsten isoenzyme of formylmethanofuran dehydrogenase in the thermophilic archaeon Methanobacterium wolfei (1992), Eur. J. Biochem., 209, 1013-1018.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | molybdenum-containing isoenzyme | Methanothermobacter wolfeii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
N-formylmethanofuran | molybdenum enzyme | Methanothermobacter wolfeii | |
| 0.035 | - |
formate | - |
Methanothermobacter wolfeii | |
| 0.037 | - |
methylviologen | molybdenum enzyme | Methanothermobacter wolfeii | |
| 0.053 | - |
N-Furfurylformamide | molybdenum enzyme | Methanothermobacter wolfeii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the molybdenum isoenzyme contains 6.6 mol non-heme iron per mol of the 146000 MW protein | Methanothermobacter wolfeii | |
| Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii | |
| Molybdenum | enzyme exists as a molybdenum containing isoenzyme and as a tungsten containing isoenzyme | Methanothermobacter wolfeii | |
| Molybdenum | molybdenum isoenzyme contains approximately 0.23 mol molybdenum and 0.46 mol molybdopterin guanine dinucleotide per mol of the 146000 MW protein | Methanothermobacter wolfeii | |
| Tungsten | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 31000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 51000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 51000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 64000 | - |
or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
| 64000 | - |
or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| 130000 | - |
native PAGE | Methanothermobacter wolfeii |
| 260000 | - |
gel filtration | Methanothermobacter wolfeii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter wolfeii | - |
a molybdenum-containing isoenzyme and a tungsten-containing isoenzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| molybdenum-containing isoenzyme | Methanothermobacter wolfeii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.3 | - |
- |
Methanothermobacter wolfeii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Formate + oxidized acceptor | - |
Methanothermobacter wolfeii | ? | - |
? | |
| formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter wolfeii | methanofuran + reduced methylviologen + CO2 | - |
? | |
| N-Furfurylformamide + oxidized acceptor | - |
Methanothermobacter wolfeii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | or trimer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDs-PAGE | Methanothermobacter wolfeii |
| trimer | or hexamer, 1 or 2 * 64000 + 1 or 2 * 51000 + 1 or 2 * 31000, SDS-PAGE | Methanothermobacter wolfeii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 7.4, thermostable up to | Methanothermobacter wolfeii |
| 65 | - |
pH 7.4, in presence of 0.1 M ammonium sulfate, 0.1 M potassium sulfate, and 0.5 M potassium chloride, thermostable up to | Methanothermobacter wolfeii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
maximal activity above | Methanothermobacter wolfeii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | no activity below pH 6.5, maximal activity above pH 7.5 | Methanothermobacter wolfeii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
