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Literature summary for 1.2.7.11 extracted from

  • Yan, Z.; Fushinobu, S.; Wakagi, T.
    Four Cys residues in heterodimeric 2-oxoacid:ferredoxin oxidoreductase are required for CoA-dependent oxidative decarboxylation but not for a non-oxidative decarboxylation (2014), Biochim. Biophys. Acta, 1844, 736-743.
    View publication on PubMed
Search for more literature for 1.2.7.11

Protein Variants

Protein Variants Comment Organism
C12/15A loss of ironĀ–sulfur cluster Sulfurisphaera tokodaii
C12A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii
C15A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii
C197A the enzyme retains an unidentified type of ironĀ–sulfur cluster Sulfurisphaera tokodaii
C46A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S center the enzyme has one [4FeĀ–4S]2+ cluster, ligated by 4 Cys residues, C12, C15, C46, and C197. All four Cys are required to fold a [4FeĀ–4S] cluster for oxidative decarboxylation of pyruvate including the formation of a stable hydroxyethyl-thiamine diphosphate radical Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin Sulfurisphaera tokodaii
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin
-
 Sulfurisphaera tokodaii acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
2-oxoacid:ferredoxin oxidoreductase
-
 Sulfurisphaera tokodaii
OFOR
-
 Sulfurisphaera tokodaii

Temperature Optimum [Ā°C]

Temperature Optimum [Ā°C] Temperature Optimum Maximum [Ā°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Sulfurisphaera tokodaii