Literature summary for 1.2.7.11 extracted from

Luo, J.; Fukuda, E.; Takase, H.; Fushinobu, S.; Shoun, H.; Wakagi, T.
Identification of the lysine residue responsible for coenzyme A binding in the heterodimeric 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus tokodaii, a thermoacidophilic archaeon, using 4-fluoro-7-nitrobenzofurazan as an affinity label (2009), Biochim. Biophys. Acta, 1794, 335-340.

Search for more literature for 1.2.7.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes K125A and K173A in Escherichia coli	Sulfurisphaera tokodaii

Protein Variants

Protein Variants	Comment	Organism
K125A	the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme	Sulfurisphaera tokodaii
K173A	the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme	Sulfurisphaera tokodaii

Inhibitors

Inhibitors	Comment	Organism	Structure
4-fluoro-7-nitrobenzofurazan	0.001 mM inhibitor with 0.01 mM enzyme decreases the activity by about 20% and 0.003 mM inhibitor with 0.01 mM enzyme decreases the activity by around 50%. Inactivation is prevented by CoA	Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	CoA	pH 8.5, 80°C, mutant enzyme K173A	Sulfurisphaera tokodaii
0.074	-	CoA	pH 8.5, 80°C, wild-type enzyme	Sulfurisphaera tokodaii
1.17	-	CoA	pH 8.5, 80°C, mutant enzyme K125A	Sulfurisphaera tokodaii

Organism

Organism	UniProt	Comment	Textmining
Sulfurisphaera tokodaii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + CoA + 2 oxidized ferredoxin	Lys125 in subunit b is the critical residue that interacts with CoA	Sulfurisphaera tokodaii	acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
StOFOR	-	Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Sulfurisphaera tokodaii

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Release 2023.1 (January 2023)