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Literature summary for 1.2.7.1 extracted from

  • Kunow, J.; Linder, D.; Thauer, R.K.
    Pyruvate:ferredoxin oxidoreductase from the sulfate-reducing Archaeoglobus fulgidus: molecular composition, catalytic properties, and sequence alignments (1995), Arch. Microbiol., 163, 21-28.
    View publication on PubMed
Search for more literature for 1.2.7.1

Inhibitors

Inhibitors Comment Organism Structure
dioxygen the enzyme is inactivated by dioxygen. Less than 50% of the original activity remains when the protein (0.23 mg/ml in 50 mM Tris/HCl pH 7.5) is exposed to air for 80 min at 25°C. Attempts to reactivate the enzyme by anoxic incubation in the presence of 2 mM dithiothreitol fail Archaeoglobus fulgidus
KCl 0.5 M, 50% inhibition Archaeoglobus fulgidus
additional information the enzyme is not inhibited by sodium nitrite (50 mM) or carbon monoxide (100% CO as gas phase) Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 oxidized ferredoxin pH 7.5, 65°C Archaeoglobus fulgidus
0.02
-
 CoA pH 7.5, 65°C Archaeoglobus fulgidus
0.3
-
 pyruvate pH 7.5, 65°C Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron per mol, the enzyme contains 9 mol of non-heme iron, and 8 mol of acid-labile sulfur. No inhibition by sodium arsenite Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
25000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
33000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
45000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
120000
-
 gel filtration Archaeoglobus fulgidus
125000
-
 polyacrylamide gradient gel electrophoresis Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is inactivated by O2. Less than 50% of the original activity remains when the protein (0.23 mg/ml in 50 mM Tris/HCl pH 7.5) is exposed to air for 80 min at 25°C. Attempts to reactivate the enzyme by anoxic incubation in the presence of 2 mM dithiothreitol fail Archaeoglobus fulgidus

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin
-
 Archaeoglobus fulgidus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
pyruvate + CoA + oxidized methyl viologen the enzyme does not catalyze the oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, and hydroxypyruvate Archaeoglobus fulgidus acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
pyruvate:ferredoxin oxidoreductase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Archaeoglobus fulgidus
90
-
 above Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 60 min, in the presence of 2 M KCl, 50% loss of activity. In absence of salt 50% loss of activity after 50 min Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate per mol, the enzyme contains 0.8 mol thiamine diphosphate Archaeoglobus fulgidus