Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylamine | little ADH I activity is detected in the absence of ethylamine or ammonium ions | Pseudomonas putida | |
additional information | ADH I is induced by ethanol | Pseudomonas putida | |
NH4+ | little ADH I activity is detected in the absence of ethylamine or ammonium ions | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | allows dissociation of ADH I into two identical monomer subunits, releasing PQQ | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas putida | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
ADH IIB, gel filtration | Pseudomonas putida |
64000 | - |
2 * 64000, ADH I, SDS-PAGE | Pseudomonas putida |
68000 | - |
ADH IIG, gel filtration | Pseudomonas putida |
69000 | - |
1 * 69000, ADH IIB | Pseudomonas putida |
72000 | - |
1 * 72000, ADH IIG | Pseudomonas putida |
130000 | - |
ADH I, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | ? | - |
? | |
additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | ? | - |
? | |
additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | ? | - |
? | |
additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | ? | - |
? | |
additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | ? | - |
? | |
additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
isozyme ADH I | - |
Pseudomonas putida | Q4W6G0 | ADH IIG; isozyme ADH IIG | - |
Pseudomonas putida | Q8GR64 | isozyme ADH IIB | - |
Pseudomonas putida HK5 | - |
isozyme ADH I | - |
Pseudomonas putida HK5 | Q4W6G0 | ADH IIG; isozyme ADH IIG | - |
Pseudomonas putida HK5 | Q8GR64 | isozyme ADH IIB | - |
Purification (Comment) | Organism |
---|---|
native isozyme ADH I, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
native isozyme ADH IIB, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
native isozyme ADH IIG, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:1,2-propanediol-grown cell | isozyme ADH IIG | Pseudomonas putida | - |
culture condition:ethanol-grown cell | isozyme ADH I | Pseudomonas putida | - |
culture condition:n-butanol-grown cell | isozyme ADH IIB | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of ADH isozymes, detailed overview | Pseudomonas putida |
14 | - |
purified ADH IIG, substrate glycerol with cofactor ferricyanide | Pseudomonas putida |
26.8 | - |
purified ADH I, substrate ethanol with cofactors | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + 2 ferricyanide + H2O | - |
Pseudomonas putida | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
acetaldehyde + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
acetaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
acetaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
acetaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
acetaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
butanal + 2 ferricyanide + H2O | - |
Pseudomonas putida | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
butanal + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
butyraldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
butyraldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
butyraldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
butyraldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | Pseudomonas putida | ? | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | Pseudomonas putida | ? | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | Pseudomonas putida | ? | - |
? | |
additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida | ? | - |
? | |
additional information | isozyme ADH IIG shows no activity with O2 | Pseudomonas putida | ? | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | Pseudomonas putida HK5 | ? | - |
? | |
additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida HK5 | ? | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | Pseudomonas putida HK5 | ? | - |
? | |
additional information | isozyme ADH IIG shows no activity with O2 | Pseudomonas putida HK5 | ? | - |
? | |
additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | Pseudomonas putida HK5 | ? | - |
? | |
propanal + 2 ferricyanide + H2O | - |
Pseudomonas putida | propanoate + 2 ferrocyanide + 2 H+ | - |
? | |
propanal + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | propanoate + 2 ferrocyanide + 2 H+ | - |
? | |
propionaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
propionaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
propionaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
propionaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 64000, ADH I, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 69000, ADH IIB | Pseudomonas putida |
monomer | 1 * 72000, ADH IIG | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ADH I | - |
Pseudomonas putida |
ADH IIB | - |
Pseudomonas putida |
ADH IIG | - |
Pseudomonas putida |
quinohemoprotein dehydrogenase | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
about, ADH IIB | Pseudomonas putida |
8 | - |
about, ADH IIG | Pseudomonas putida |
9 | - |
about, ADH I | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | isozyme ADH IIB is a quinohemoprotein dehydrogenase, determination of heme content, overview | Pseudomonas putida | |
heme c | isozymes ADH IIB and ADH IIG are quinohemoprotein dehydrogenases, while ADH I is a non-heme quinoprotein, determination of heme contents, overview | Pseudomonas putida | |
additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida | |
pyrroloquinoline quinone | PQQ, a prosthetic group, ADH I is a non-heme quinoprotein containing 2.2 mol of PQQ per mol of enzyme | Pseudomonas putida | |
pyrroloquinoline quinone | PQQ, a prosthetic group, isozyme ADH IIB is a quinohemoprotein dehydrogenase. ADH IIB contains 1.4 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da | Pseudomonas putida | |
pyrroloquinoline quinone | PQQ, a prosthetic group, isozyme ADH IIG is a quinohemoprotein dehydrogenase. ADH IIG contains 1.2 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da | Pseudomonas putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas putida | ADH IIG, isoelectric focusing | - |
5 |
Pseudomonas putida | ADH IIB, isoelectric focusing | - |
6.5 |
Pseudomonas putida | ADH I, isoelectric focusing | - |
7.6 |