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Literature summary for 1.2.5.2 extracted from

  • Gomez-Manzo, S.; Contreras-Zentella, M.; Gonzalez-Valdez, A.; Sosa-Torres, M.; Arreguin-Espinoza, R.; Escamilla-Marvan, E.
    The PQQ-alcohol dehydrogenase of Gluconacetobacter diazotrophicus (2008), Int. J. Food Microbiol., 125, 71-78.
    View publication on PubMed
Search for more literature for 1.2.5.2

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
71400
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
115000
-
 non-denaturing PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + ubiquinone Gluconacetobacter diazotrophicus electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone acetate + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
258
-
-
 Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2 ferricyanide + H2O 87% of the activity with allyl alcohol Gluconacetobacter diazotrophicus acetate + 2 ferrocyanide + 2 H+
-
 ?
acetaldehyde + ubiquinone electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone Gluconacetobacter diazotrophicus acetate + ubiquinol
-
 ?
glutaraldehyde + 4 ferricyanide + 2 H2O 25% of the activity with allyl alcohol Gluconacetobacter diazotrophicus glutarate + 4 ferrocyanide + 4 H+
-
 ?
additional information no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol Gluconacetobacter diazotrophicus ?
-
 ?
propanal + 2 ferricyanide + H2O 42% of the activity with allyl alcohol Gluconacetobacter diazotrophicus propanoate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, 80% loss of activity Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate: acetaldehyde Gluconacetobacter diazotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: acetaldehyde Gluconacetobacter diazotrophicus

Cofactor

Cofactor Comment Organism Structure
cytochrome c presence of cytochrome c in both subunits Gluconacetobacter diazotrophicus
pyrroloquinoline quinone electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone Gluconacetobacter diazotrophicus

pI Value

Organism Comment pI Value Maximum pI Value
Gluconacetobacter diazotrophicus gradient electrophoresis, determined in pH range 3.4-9.0
-
 6.1