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Literature summary for 1.2.5.2 extracted from

  • Groen, B.W.; van Kleef, M.A.G.; Duine, J.A.
    Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni (1986), Biochem. J., 234, 611-615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.5.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
apoenzyme Comamonas testosteroni

Storage Stability

Storage Stability Organism
-70°C, stable for prolonged periods Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + oxidized tetramethylphenylenediamine
-
 Comamonas testosteroni acetate + reduced tetramethylphenylenediamine
-
 ?
butyraldehyde + oxidized tetramethylphenylenediamine
-
 Comamonas testosteroni butanoate + reduced tetramethylphenylenediamine
-
 ?
formaldehyde + oxidized tetramethylphenylenediamine
-
 Comamonas testosteroni formate + reduced tetramethylphenylenediamine
-
 ?
additional information primary alcohols (except methanol), secondary alcohols and aldehydes are substrates, and a broad range of dyes functions as artificial electron acceptor Comamonas testosteroni ?
-
 ?
octanal + oxidized tetramethylphenylenediamine
-
 Comamonas testosteroni octanoate + reduced tetramethylphenylenediamine
-
 ?

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains one heme c group, and full reconstitution is achieved with 1 mol of pyrroloquinoline quinone/mol Comamonas testosteroni
pyrroloquinoline quinone after reconstitution to the holoenzyme by the addition of pyrroloquinoline quinone, addition of substrate changes the absorption spectrum to that of reduced cytochrome c, indicating that the heme c group participates in the enzymic mechanism, one molecule of pyrroloquinoline quinone is bound per enzyme molecule Comamonas testosteroni