Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D108N | less thermostable than wild type enzyme | Homo sapiens |
D200A | less thermostable than wild type enzyme | Homo sapiens |
D200A/D108N | the mutant is defective in folding and assembly and as no detectable overall activity | Homo sapiens |
D200N | less thermostable than wild type enzyme | Homo sapiens |
E198Q/D200N | the mutant is defective in folding and assembly and as no detectable overall activity | Homo sapiens |
additional information | the hybrid E1b heterotetramer carrying one wild type, one S292Qalpha subunit, and two wild type beta subunits contains only one functional active site and exhibits nearly 40% of the wild type activity | Homo sapiens |
S292Q | no activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | branched-chain alpha-keto acid dehydrogenase overall activity is nullified at 50% phosphorylation of E1b | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.119 | - |
2-oxo-isovalerate | wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His Trap HP column chromatography and Strep-Tactin Superflow gravity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate + 2,6-dichlorophenolindophenol + CoA | - |
Homo sapiens | ? + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
BCKDC | - |
Homo sapiens |
branched-chain alpha-keto acid dehydrogenase | - |
Homo sapiens |
E1b component | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.4 | - |
2-oxo-isovalerate | wild type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Homo sapiens |