Protein Variants | Comment | Organism |
---|---|---|
D549A | in the mutant, binding and reductive acetylation of E2p lipoyl domains are highly impaired | Escherichia coli |
E401K | in the mutant, binding and reductive acetylation of E2p lipoyl domains are highly impaired | Escherichia coli |
H407A | in the mutant, binding and reductive acetylation of E2p lipoyl domains are highly impaired | Escherichia coli |
Y177A | in the mutant, binding and reductive acetylation of E2p lipoyl domains are highly impaired | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
E2p lipoyl domain | wild type enzyme, at pH 7.0 and 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99474 | - |
x * 99474, calculated from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + E2p lipoyl domain | - |
Escherichia coli | acetylated E2p lipoyl domain + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99474, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
E1p | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
E2p lipoyl domain | mutant enzyme H407A, at pH 7.0 and 30°C | Escherichia coli | |
0.7 | - |
E2p lipoyl domain | mutant enzyme D549A, at pH 7.0 and 30°C | Escherichia coli | |
0.83 | - |
E2p lipoyl domain | mutant enzyme E401K, at pH 7.0 and 30°C | Escherichia coli | |
3.31 | - |
E2p lipoyl domain | mutant enzyme Y177A, at pH 7.0 and 30°C | Escherichia coli | |
95 | - |
E2p lipoyl domain | wild type enzyme, at pH 7.0 and 30°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Escherichia coli |